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Conboy JJ, Henion JD. The determination of glycopeptides by liquid chromatography/mass spectrometry with collision-induced dissociation. J Am Soc Mass Spectrom. 1992;3(8):804-14doi: 10.1016/1044-0305(92)80003-4.
Conboy, J. J., & Henion, J. D. (1992). The determination of glycopeptides by liquid chromatography/mass spectrometry with collision-induced dissociation. Journal of the American Society for Mass Spectrometry, 3(8), 804-14. https://doi.org/10.1016/1044-0305(92)80003-4
Conboy, J J, and Henion, J D. "The determination of glycopeptides by liquid chromatography/mass spectrometry with collision-induced dissociation." Journal of the American Society for Mass Spectrometry vol. 3,8 (1992): 804-14. doi: https://doi.org/10.1016/1044-0305(92)80003-4
Conboy JJ, Henion JD. The determination of glycopeptides by liquid chromatography/mass spectrometry with collision-induced dissociation. J Am Soc Mass Spectrom. 1992 Nov;3(8):804-14. doi: 10.1016/1044-0305(92)80003-4. PMID: 24234703.
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J Am Soc Mass Spectrom. 1992 Nov;3(8):804-14. doi: 10.1016/1044-0305(92)80003-4.

The determination of glycopeptides by liquid chromatography/mass spectrometry with collision-induced dissociation.

Journal of the American Society for Mass Spectrometry

J J Conboy, J D Henion

Affiliations

  1. Drug Testing and Toxicology, New York State College of Veterinary Medicine, Cornell University, 925 Warren, 14850, Ithaca, New York, USA.

PMID: 24234703 DOI: 10.1016/1044-0305(92)80003-4

Abstract

Glycopeptides derived from ribonuclease B and ovomucoid have been subjected to collisioninduced dissociation (CID) in the second quadrupole of a triple quadrupole mass spectrometer. Doubly charged parent ions gave predictable fragmentation that yielded partial sequence information of the attached oligosaccharide as Hex and HexNAc units. Common oxonium ions are observed in the product ion mass spectra of the glycopeptides that correspond to HexNAc(+) (m/z 204) and HexHexNAc(+) (m/z 366). A strategy for locating the glycopeptides in the proteolytic digest mixtures of glycoproteins by ions spray liquid chromatography mass spectrometry (LC/MS) is described by utilizing CID in the declustering region of the atmospheric pressure ionization mass spectrometer to produce these characteristic oxonium ions. This LC/CID/MS approach is used to identify glycopeptides in proteolytic digest mixtures of ovomucoid, asialofetuin, and fetuin. LC/CID/MS in the selected ion monitoring mode may be used to identify putative glycopeptides from the proteolytic digest of fetuin.

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