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Maan AC, Vreugdenhil D, Bogers RJ, et al. The complex kinetics of auxin-binding to a particulate fraction from tobacco-pith callus. Planta. 1983;158(1):10-5doi: 10.1007/BF00395397.
Maan, A. C., Vreugdenhil, D., Bogers, R. J., & Libbenga, K. R. (1983). The complex kinetics of auxin-binding to a particulate fraction from tobacco-pith callus. Planta, 158(1), 10-5. https://doi.org/10.1007/BF00395397
Maan, A C, et al. "The complex kinetics of auxin-binding to a particulate fraction from tobacco-pith callus." Planta vol. 158,1 (1983): 10-5. doi: https://doi.org/10.1007/BF00395397
Maan AC, Vreugdenhil D, Bogers RJ, Libbenga KR. The complex kinetics of auxin-binding to a particulate fraction from tobacco-pith callus. Planta. 1983 Jun;158(1):10-5. doi: 10.1007/BF00395397. PMID: 24264442.
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Planta. 1983 Jun;158(1):10-5. doi: 10.1007/BF00395397.

The complex kinetics of auxin-binding to a particulate fraction from tobacco-pith callus.

Planta

A C Maan, D Vreugdenhil, R J Bogers, K R Libbenga

Affiliations

  1. Botanisch Laboratorium, Rijksuniversiteit Leiden, Nonnensteeg 3, NL-2311 VJ, Leiden, The Netherlands.

PMID: 24264442 DOI: 10.1007/BF00395397

Abstract

The kinetics of binding of 1-naphthylacetic acid to particulate fractions from tobacco-pith callus were studied. This binding site does not bind auxin at 0° C. Binding experiments performed at 25° C demonstrated an apparent K a of approx. 6.5·10(6) M(-1). A filtration method was developed in order to study non-equilibrium kinetics of this binding. Dissociation of the complex of auxin and binding site indicates the presence of at least two binding components with dissociation rate constants (k off) of 6.1·10(-3) min(-1) and 6.0·10(-2) min(-1). This binding behaviour was not independent, indicating that the binding of auxin to the particulate fractions was more complex than binding of one hormone molecule to one binding site. This complexity was further confirmed by experiments in which the initial velocity of complex formation was measured. A model was worked out into which our data fit without contradictions. It involves the binding of four hormone molecules to one receptor molecule.

References

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