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Laulhé S, Geers TE, Shi X, et al. Electron Ionization-Induced Release of Coded Isotopic Reporter Ions in an . Anal Methods. 2013;5(18)doi: 10.1039/C3AY41124A.
Laulhé, S., Geers, T. E., Shi, X., Zhang, X., & Nantz, M. H. (2013). Electron Ionization-Induced Release of Coded Isotopic Reporter Ions in an . Analytical methods : advancing methods and applications, 5(18), . https://doi.org/10.1039/C3AY41124A
Laulhé, Sébastien, et al. "Electron Ionization-Induced Release of Coded Isotopic Reporter Ions in an ." Analytical methods : advancing methods and applications vol. 5,18 (2013). doi: https://doi.org/10.1039/C3AY41124A
Laulhé S, Geers TE, Shi X, Zhang X, Nantz MH. Electron Ionization-Induced Release of Coded Isotopic Reporter Ions in an . Anal Methods. 2013 Sep 21;5(18). doi: 10.1039/C3AY41124A. PMID: 24235976; PMCID: PMC3822575.
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Anal Methods. 2013 Sep 21;5(18). doi: 10.1039/C3AY41124A.

Electron Ionization-Induced Release of Coded Isotopic Reporter Ions in an .

Analytical methods : advancing methods and applications

Sébastien Laulhé, Tyler E Geers, Xue Shi, Xiang Zhang, Michael H Nantz

Affiliations

  1. University of Louisville, Department of Chemistry, 2320 South Brook Street, Louisville, Kentucky 40292, United States. Tel: 01 502 852 8069.

PMID: 24235976 PMCID: PMC3822575 DOI: 10.1039/C3AY41124A

Abstract

We describe an isotope coding strategy that enables simultaneous GC-MS analysis of multiple samples for substrate identification and quantification. The method relies on direct measurement of isotopic ethyl carbenium ions serving as mass spectral tags in a zone of minimal interference (ZMI) at m/z 31-37. Sample aldehyde and ketone mixtures were reacted with isotopic 2-aminooxyethyl propionates to illustrate the method, which determined the relative abundance of the mixed compounds with an average 95% accuracy. ZMI reporter ion detection also enables chemoselective substrate profiling and absolute quantification, as demonstrated using a biologically derived sample.

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