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Kost B, Potrykus I, Neuhaus G. Regeneration of fertile plants from excised immature zygotic embryos of Arabidopsis thaliana. Plant Cell Rep. 1992;12(1):50-4doi: 10.1007/BF00232423.
Kost, B., Potrykus, I., & Neuhaus, G. (1992). Regeneration of fertile plants from excised immature zygotic embryos of Arabidopsis thaliana. Plant cell reports, 12(1), 50-4. https://doi.org/10.1007/BF00232423
Kost, B, et al. "Regeneration of fertile plants from excised immature zygotic embryos of Arabidopsis thaliana." Plant cell reports vol. 12,1 (1992): 50-4. doi: https://doi.org/10.1007/BF00232423
Kost B, Potrykus I, Neuhaus G. Regeneration of fertile plants from excised immature zygotic embryos of Arabidopsis thaliana. Plant Cell Rep. 1992 Dec;12(1):50-4. doi: 10.1007/BF00232423. PMID: 24201731.
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Plant Cell Rep. 1992 Dec;12(1):50-4. doi: 10.1007/BF00232423.

Regeneration of fertile plants from excised immature zygotic embryos of Arabidopsis thaliana.

Plant cell reports

B Kost, I Potrykus, G Neuhaus

Affiliations

  1. Institute for Plant Sciences, Swiss Federal Institute of Technology, Universitätsstrasse 2, CH-8092, Zürich, Switzerland.

PMID: 24201731 DOI: 10.1007/BF00232423

Abstract

We present protocols for the regeneration of fertile plants from immature zygotic embryos of Arabidopsis thaliana ecotype "Zürich" either directly by multiple shoot formation from the apical region or indirectly by shoot induction from embryo derived calli. The regeneration efficiency by "multiple shooting" depended on the developmental stage of the cultured embryos and ranged from 15% for early heart shaped to 90% for early torpedo shaped and further developed embryos. 85% callus induction was achieved from embryos in the early torpedo shaped or a later stage of development. The efficiency of shoot induction from embryo derived calli varied between 25% and 75% in different experiments.

References

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