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Walden R, Czaja I, Schmülling T, et al. Rol genes alter hormonal requirements for protoplast growth and modify the expression of an auxin responsive promoter. Plant Cell Rep. 1993;12(10):551-4doi: 10.1007/BF00233058.
Walden, R., Czaja, I., Schmülling, T., & Schell, J. (1993). Rol genes alter hormonal requirements for protoplast growth and modify the expression of an auxin responsive promoter. Plant cell reports, 12(10), 551-4. https://doi.org/10.1007/BF00233058
Walden, R, et al. "Rol genes alter hormonal requirements for protoplast growth and modify the expression of an auxin responsive promoter." Plant cell reports vol. 12,10 (1993): 551-4. doi: https://doi.org/10.1007/BF00233058
Walden R, Czaja I, Schmülling T, Schell J. Rol genes alter hormonal requirements for protoplast growth and modify the expression of an auxin responsive promoter. Plant Cell Rep. 1993 Aug;12(10):551-4. doi: 10.1007/BF00233058. PMID: 24201783.
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Plant Cell Rep. 1993 Aug;12(10):551-4. doi: 10.1007/BF00233058.

Rol genes alter hormonal requirements for protoplast growth and modify the expression of an auxin responsive promoter.

Plant cell reports

R Walden, I Czaja, T Schmülling, J Schell

Affiliations

  1. Max-Planck-Institut für Züchtungsforschung, Carl von Linne Weg 10, D-50837, Köln, Germany.

PMID: 24201783 DOI: 10.1007/BF00233058

Abstract

Growth characteristics of tobacco protoplasts containing rolA linked to its own promoter, or the rolB, or rolC genes of Agrobacterium rhizogenes linked to the Cauliflower Mosaic Virus 35S RNA promoter were compared with those from untransformed plants. RolA protoplasts require auxin and cytokinin for callus formation. Protoplasts overexpressing rolB and C form callus in the absence of exogenously applied auxin and cytokinin, respectively. Long term callus growth requires auxin, but the requirement for cytokinin is not critical. Optimal transient expression of an auxin responsive promoter element occurred at lower external levels of auxin in rolB and rolC protoplasts compared with untransformed protoplasts. Addition of putrescine was required for auxin responsive transient gene expression in rolA protoplasts suggesting that polyamines, or their products affect gene expression in rolA plants.

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