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Kamp-Glass M, Powell D, Reddy GB, et al. Biotechniques for improving acid aluminum tolerance in alfalfa. Plant Cell Rep. 1993;12(10):590-2doi: 10.1007/BF00233067.
Kamp-Glass, M., Powell, D., Reddy, G. B., Baligar, V. C., & Wright, R. J. (1993). Biotechniques for improving acid aluminum tolerance in alfalfa. Plant cell reports, 12(10), 590-2. https://doi.org/10.1007/BF00233067
Kamp-Glass, M, et al. "Biotechniques for improving acid aluminum tolerance in alfalfa." Plant cell reports vol. 12,10 (1993): 590-2. doi: https://doi.org/10.1007/BF00233067
Kamp-Glass M, Powell D, Reddy GB, Baligar VC, Wright RJ. Biotechniques for improving acid aluminum tolerance in alfalfa. Plant Cell Rep. 1993 Aug;12(10):590-2. doi: 10.1007/BF00233067. PMID: 24201792.
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Plant Cell Rep. 1993 Aug;12(10):590-2. doi: 10.1007/BF00233067.

Biotechniques for improving acid aluminum tolerance in alfalfa.

Plant cell reports

M Kamp-Glass, D Powell, G B Reddy, V C Baligar, R J Wright

Affiliations

  1. N.C. A & T State University, 1601 E. Market St., 27411, Greensboro, N.C., USA.

PMID: 24201792 DOI: 10.1007/BF00233067

Abstract

Alfalfa (Medicago sativa L.), cultivars ARC, Regen Y and Saranac were selected in vitro in a recently developed acid/aluminum toxic media. The new media produced higher initiation rates and higher fresh callus weights than those obtainable with the media described by Meredith and Connor for the selection of aluminum resistant variants in Nicotiana plumbaginofolia. Both rescue and direct initiation yielded adequate amounts of healthy callus for the initiation of embryogenesis. The toxic effect of the acid/aluminum media is expressed in both the percent of explants initiating callus and in the fresh-weights obtained during initiation and two subsequent subcultures.

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