Display options
Cite
Hobbs SL, Pelcher LE, Delong CM, et al. Genetic variability in the amount of ribulose-1,5-bisphosphate carboxylase/oxygenase and its small subunit mRNA in pea. Planta. 1990;180(4):510-6doi: 10.1007/BF02411448.
Hobbs, S. L., Pelcher, L. E., Delong, C. M., Anderson, M., & Mahon, J. D. (1990). Genetic variability in the amount of ribulose-1,5-bisphosphate carboxylase/oxygenase and its small subunit mRNA in pea. Planta, 180(4), 510-6. https://doi.org/10.1007/BF02411448
Hobbs, S L, et al. "Genetic variability in the amount of ribulose-1,5-bisphosphate carboxylase/oxygenase and its small subunit mRNA in pea." Planta vol. 180,4 (1990): 510-6. doi: https://doi.org/10.1007/BF02411448
Hobbs SL, Pelcher LE, Delong CM, Anderson M, Mahon JD. Genetic variability in the amount of ribulose-1,5-bisphosphate carboxylase/oxygenase and its small subunit mRNA in pea. Planta. 1990 Mar;180(4):510-6. doi: 10.1007/BF02411448. PMID: 24202095.
Copy Download .nbib Format: NLM
Share it on

Planta. 1990 Mar;180(4):510-6. doi: 10.1007/BF02411448.

Genetic variability in the amount of ribulose-1,5-bisphosphate carboxylase/oxygenase and its small subunit mRNA in pea.

Planta

S L Hobbs, L E Pelcher, C M Delong, M Anderson, J D Mahon

Affiliations

  1. Plant Biotechnology Institute, National Research Council of Canada, 110 Gymnasium Road, S7N OW9, Saskatoon, Sask, Canada.

PMID: 24202095 DOI: 10.1007/BF02411448

Abstract

The amount of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco; EC 4.1.1.39) was studied during greening in 12Pisum sativum L. genotypes. The proportion of mRNA coding for the small subunit of Rubisco (SSU mRNA) was also monitored by hybridization with a cDNA (complementary DNA) probe from one of the nuclear genes coding for SSU (rbcS). Both the SSU mRNA and Rubisco (in g·FW)(-1)) contents rapidly increased in all genotypes on illumination of dark-grown seedlings. Natural genetic variability was found in the amounts of SSU mRNA, Rubisco· (g FW)(-1), total RNA·(g FW)(-1) and mRNA·(μg total RNA)(-1). Differences among genotypes in SSU mRNA were apparently the result of differences in the rate of light-induced accumulation of therbcS gene transcripts. Genotype means for SSU mRNA and Rubisco· (g FW)(-1) amounts during greening were significantly correlated (r=0.788;P<0.01). This indicates a relationship between genetic differences in the rate of light-induced accumulation of therbcS gene transcripts and the Rubisco amount, and establishes a link between natural genetic variability at the molecular and the physiological levels. Genotypic variability in SSU mRNA during greening was also positively correlated to the Rubisco content per unit leaf area in fully greened leaves. Although Southern-blot analysis indicated that there was also natural genetic variability in the copy number of therbcS genes, this difference in copy number could not account for the differences in SSU mRNA production.

References

  1. EMBO J. 1985 Dec 1;4(12):3055-61 - PubMed
  2. J Biol Chem. 1983 Feb 10;258(3):1399-402 - PubMed
  3. Science. 1986 May 30;232(4754):1106-12 - PubMed
  4. Proc Natl Acad Sci U S A. 1985 Aug;82(15):5083-7 - PubMed
  5. EMBO J. 1985 Dec 1;4(12):3063-8 - PubMed
  6. Mol Gen Genet. 1987 Sep;209(2):247-56 - PubMed
  7. Plant Physiol. 1987 Aug;84(4):1238-43 - PubMed
  8. EMBO J. 1986 Sep;5(9):2063-2071 - PubMed
  9. Scand J Immunol Suppl. 1973;1:15-35 - PubMed
  10. Cell. 1988 Nov 18;55(4):673-81 - PubMed
  11. EMBO J. 1984 Aug;3(8):1671-9 - PubMed
  12. Nucleic Acids Res. 1987 Nov 11;15(21):8661-77 - PubMed
  13. Plant Mol Biol. 1987 Sep;8(5):375-82 - PubMed
  14. J Biol Chem. 1981 Mar 10;256(5):2315-20 - PubMed

Publication Types