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Ponchautcor S, Veitchaff K, Libertaff R, et al. Analysis by fast-atom bombardment tandem mass spectrometry of phosphatidylcholine isolated from heart mitochondrial fractions: Evidence of incorporation of monohydroxylated fatty acyl moieties. J Am Soc Mass Spectrom. 1996;7(1):50-8doi: 10.1016/1044-0305(95)00623-0.
Ponchautcor, S., Veitchaff, K., Libertaff, R., Van Hoofaff, F., Hueaff, L., & de Hoffmann, E. (1996). Analysis by fast-atom bombardment tandem mass spectrometry of phosphatidylcholine isolated from heart mitochondrial fractions: Evidence of incorporation of monohydroxylated fatty acyl moieties. Journal of the American Society for Mass Spectrometry, 7(1), 50-8. https://doi.org/10.1016/1044-0305(95)00623-0
Ponchautcor, S, et al. "Analysis by fast-atom bombardment tandem mass spectrometry of phosphatidylcholine isolated from heart mitochondrial fractions: Evidence of incorporation of monohydroxylated fatty acyl moieties." Journal of the American Society for Mass Spectrometry vol. 7,1 (1996): 50-8. doi: https://doi.org/10.1016/1044-0305(95)00623-0
Ponchautcor S, Veitchaff K, Libertaff R, Van Hoofaff F, Hueaff L, de Hoffmann E. Analysis by fast-atom bombardment tandem mass spectrometry of phosphatidylcholine isolated from heart mitochondrial fractions: Evidence of incorporation of monohydroxylated fatty acyl moieties. J Am Soc Mass Spectrom. 1996 Jan;7(1):50-8. doi: 10.1016/1044-0305(95)00623-0. PMID: 24202794.
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J Am Soc Mass Spectrom. 1996 Jan;7(1):50-8. doi: 10.1016/1044-0305(95)00623-0.

Analysis by fast-atom bombardment tandem mass spectrometry of phosphatidylcholine isolated from heart mitochondrial fractions: Evidence of incorporation of monohydroxylated fatty acyl moieties.

Journal of the American Society for Mass Spectrometry

S Ponchautcor, K Veitchaff, R Libertaff, F Van Hoofaff, L Hueaff, E de Hoffmann

Affiliations

  1. Hormone and Metabolic Research Unit, Laboratory of Metabolic Diseases, International Institute of Cellular and Molecular Pathology, Belgium.

PMID: 24202794 DOI: 10.1016/1044-0305(95)00623-0

Abstract

Phosphatidylcholine (PC) is one of the main phospholipids present in mitochondrial membranes. According to current knowledge, the predominant fatty acyl moieties in this phospholipid are 16, 18, 20, or 22 carbon atoms long with chains that contain only carbon and hydrogen atoms. We have conducted a detailed analysis of the fatty acid substituents of the phospholipids present in mitochondrial fractions by using fast-atom bombardment tandem PC extracted from mitochondrial fractions of rat heart. The structure of one of these monohydroxylated fatty acids has been elucidated and corresponded to 12-hydroxy 9-octadecenoic acid. Indications that concern the structure of the five other monohydroxylated fatty acids are presented. These monohydroxylated fatty acyl groups are preferentially associated in the PC molecule with C-18 and C-20 fatty acyl moieties. We present arguments to suggest that the formation of these compounds is probably not due to a free-radical initiated mechanism. The potential implication of these monohydroxylated fatty acids in several physiological functions is suggested by the fact that free hydroxylated fatty acids that are identical or closely related to those found in the mitochondrial fractions possess various biological activities.

References

  1. J Lipid Res. 1964 Oct;5:600-8 - PubMed
  2. J Am Soc Mass Spectrom. 1992 Jan;3(1):71-8 - PubMed
  3. Blood. 1993 Jun 15;81(12):3303-12 - PubMed
  4. Free Radic Res Commun. 1991;12-13 Pt 2:681-9 - PubMed
  5. Prostaglandins. 1991 Jan;41(1):21-7 - PubMed
  6. Biochim Biophys Acta. 1985 Jun 12;822(1):1-42 - PubMed
  7. Biochem J. 1992 Feb 1;281 ( Pt 3):709-15 - PubMed
  8. J Am Soc Mass Spectrom. 1991 Jan;2(1):45-54 - PubMed
  9. Biochem J. 1990 Jan 1;265(1):79-85 - PubMed
  10. Proc Natl Acad Sci U S A. 1990 Aug;87(15):5706-9 - PubMed
  11. Biochem Pharmacol. 1988 Apr 1;37(7):1399-405 - PubMed
  12. Arch Biochem Biophys. 1981 Oct 15;211(2):674-82 - PubMed
  13. Biochem Pharmacol. 1994 Feb 11;47(4):742-4 - PubMed
  14. N Engl J Med. 1985 Jan 17;312(3):159-63 - PubMed
  15. Eur J Pharmacol. 1993 Oct 12;243(1):87-90 - PubMed
  16. J Biol Chem. 1957 May;226(1):497-509 - PubMed
  17. Anal Chem. 1991 May 15;63(10):1032-8 - PubMed
  18. Drugs Exp Clin Res. 1991;17(7):351-3 - PubMed
  19. Lab Invest. 1982 Nov;47(5):412-26 - PubMed
  20. J Biol Chem. 1959 Mar;234(3):466-8 - PubMed
  21. Anal Biochem. 1994 Aug 15;221(1):16-24 - PubMed
  22. J Biol Chem. 1985 Dec 25;260(30):16056-9 - PubMed
  23. Biochem Biophys Res Commun. 1987 Jul 31;146(2):502-9 - PubMed
  24. Biol Mass Spectrom. 1993 Aug;22(8):465-73 - PubMed
  25. Anal Chem. 1991 Jun 1;63(11):1110-4 - PubMed
  26. J Lipid Res. 1993 Jul;34(7):1229-35 - PubMed
  27. Lipids. 1986 Sep;21(9):580-8 - PubMed
  28. Lipids. 1991 Dec;26(12):1112-6 - PubMed
  29. Anal Biochem. 1991 Oct;198(1):203-11 - PubMed
  30. Br J Pharmacol. 1992 Oct;107(2):597-603 - PubMed
  31. Prostaglandins. 1985 Jul;30(1):119-24 - PubMed
  32. Lancet. 1984 Jun 23;1(8391):1396-7 - PubMed

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