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King J, Horsch RB, Savage AD. Partial characterization of two stable auxotrophic cell strains of Datura innoxia Mill. Planta. 1980;149(5):480-4doi: 10.1007/BF00385752.
King, J., Horsch, R. B., & Savage, A. D. (1980). Partial characterization of two stable auxotrophic cell strains of Datura innoxia Mill. Planta, 149(5), 480-4. https://doi.org/10.1007/BF00385752
King, J, et al. "Partial characterization of two stable auxotrophic cell strains of Datura innoxia Mill." Planta vol. 149,5 (1980): 480-4. doi: https://doi.org/10.1007/BF00385752
King J, Horsch RB, Savage AD. Partial characterization of two stable auxotrophic cell strains of Datura innoxia Mill. Planta. 1980 Oct;149(5):480-4. doi: 10.1007/BF00385752. PMID: 24306477.
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Planta. 1980 Oct;149(5):480-4. doi: 10.1007/BF00385752.

Partial characterization of two stable auxotrophic cell strains of Datura innoxia Mill.

Planta

J King, R B Horsch, A D Savage

Affiliations

  1. Department of Biology, University of Saskatchewan, S7N 0W0, Saskatoon, Sask, Canada.

PMID: 24306477 DOI: 10.1007/BF00385752

Abstract

A growth analysis of several presumptive "leaky" auxotrophs from Datura innoxia suspension cultures led to the discovery of an adenine-requiring cultures led to the discovery of an adenine-requiring cell strain (Ad1). Both Ad1 and Pn1, a pantothenate-requiring strain isolated earlier from these cultures, still require either adenine or pantothenate for growth after more than one year in culture. Attempts to select prototrophic revertants have failed. Ad 1 also grew well in a medium containing either 5-aminoimidazole-4-carboxamide ribotide or inosine instead of adenine; Pn 1 with pantoic acid alone but not at all in the presence of β-alanine or α-ketoisovalerate alone instead of pantothenate. Pn 1 cells starved of pantothenate for up to 4d and Ad1 of adenine for 10d or more resumed growth when transferred to appropriately supplemented media. Wild-type Datura cells grown on unsupplemented medium would not crossfeed the required nutrients to the auxotrophs. The starvation and cross-feeding experiment showed that both auxotrophs could be used in reconstruction experiments to develop enrichment-selection techniques for the isolation of more auxotrophs.

References

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