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Ohtani T, Kiyokawa S, Ohgawara T, et al. Nucleotide sequences and stability of a Nicotiana nuclear DNA segment possessing autonomously replicating ability in yeast. Plant Mol Biol. 1985;5(1):35-9doi: 10.1007/BF00017871.
Ohtani, T., Kiyokawa, S., Ohgawara, T., Harada, H., & Uchimiya, H. (1985). Nucleotide sequences and stability of a Nicotiana nuclear DNA segment possessing autonomously replicating ability in yeast. Plant molecular biology, 5(1), 35-9. https://doi.org/10.1007/BF00017871
Ohtani, T, et al. "Nucleotide sequences and stability of a Nicotiana nuclear DNA segment possessing autonomously replicating ability in yeast." Plant molecular biology vol. 5,1 (1985): 35-9. doi: https://doi.org/10.1007/BF00017871
Ohtani T, Kiyokawa S, Ohgawara T, Harada H, Uchimiya H. Nucleotide sequences and stability of a Nicotiana nuclear DNA segment possessing autonomously replicating ability in yeast. Plant Mol Biol. 1985 Jan;5(1):35-9. doi: 10.1007/BF00017871. PMID: 24306538.
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Plant Mol Biol. 1985 Jan;5(1):35-9. doi: 10.1007/BF00017871.

Nucleotide sequences and stability of a Nicotiana nuclear DNA segment possessing autonomously replicating ability in yeast.

Plant molecular biology

T Ohtani, S Kiyokawa, T Ohgawara, H Harada, H Uchimiya

Affiliations

  1. Institute of Biological Sciences, University of Tsukuba, Sakura-mura, 305, Ibaraki, Japan.

PMID: 24306538 DOI: 10.1007/BF00017871

Abstract

The nucleotide sequence of a tobacco (Nicotiana tabacum) chromosomal DNA segment(t3-ars) capable of replication in yeast (ars: autonomously replicating sequences) is presented. The subcloned region (618 bp) contained 11 bp consensus (5' A/TTTTATPuTTTA/T 3') essential for several yeast ars, and 73% A and T. Unique 70 bp repetitive sequences resided next to this sequence. Thirty-two bp AT repeats were also seen in the neighbourhood of the repetitive sequence. The hybrid plasmid containing t3-ars was mitotically stabilized by the help of yeast centromere (CEN4).

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