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Ooms G, Lenton JR. T-DNA genes to study plant development: precocious tuberisation and enhanced cytokinins in A. tumefaciens transformed potato. Plant Mol Biol. 1985;5(4):205-12doi: 10.1007/BF00020638.
Ooms, G., & Lenton, J. R. (1985). T-DNA genes to study plant development: precocious tuberisation and enhanced cytokinins in A. tumefaciens transformed potato. Plant molecular biology, 5(4), 205-12. https://doi.org/10.1007/BF00020638
Ooms, G, and Lenton, J R. "T-DNA genes to study plant development: precocious tuberisation and enhanced cytokinins in A. tumefaciens transformed potato." Plant molecular biology vol. 5,4 (1985): 205-12. doi: https://doi.org/10.1007/BF00020638
Ooms G, Lenton JR. T-DNA genes to study plant development: precocious tuberisation and enhanced cytokinins in A. tumefaciens transformed potato. Plant Mol Biol. 1985 Jul;5(4):205-12. doi: 10.1007/BF00020638. PMID: 24306761.
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Plant Mol Biol. 1985 Jul;5(4):205-12. doi: 10.1007/BF00020638.

T-DNA genes to study plant development: precocious tuberisation and enhanced cytokinins in A. tumefaciens transformed potato.

Plant molecular biology

G Ooms, J R Lenton

Affiliations

  1. Biochemistry Department, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts, U.K..

PMID: 24306761 DOI: 10.1007/BF00020638

Abstract

Potato Line Mb1501B is a derivative of the cultivar Maris Bard (Solanum tuberosum), transformed with T-DNA from A. tumefaciens strain LBA1501. In culture it grew as frequently branching stunted shoots with a basal callus, lacking roots. These shoots did not form tubers. When grafted, Mb1501B shoots gradually became morphologically more normal and aerial tubers formed readily. Cultured Mb1501B shoots contained 100-200-fold higher concentrations of the biologically-active cytokinins zeatin, zeatin riboside and their corresponding side-chain o-glucosides than untransformed Maris Bard shoots. Cultured Mb1501B shoots contained approximately a 3-fold lower concentration of indole acetic acid (IAA). In grafted Mb1501B plants a 3-10-fold higher concentration of the active cytokinins was found compared with untransformed plants and no difference in IAA concentration.

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