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Bourgkard F, Favre JM. Somatic embryos from callus of Sequoia sempervirens. Plant Cell Rep. 1988;7(6):445-8doi: 10.1007/BF00269534.
Bourgkard, F., & Favre, J. M. (1988). Somatic embryos from callus of Sequoia sempervirens. Plant cell reports, 7(6), 445-8. https://doi.org/10.1007/BF00269534
Bourgkard, F, and Favre, J M. "Somatic embryos from callus of Sequoia sempervirens." Plant cell reports vol. 7,6 (1988): 445-8. doi: https://doi.org/10.1007/BF00269534
Bourgkard F, Favre JM. Somatic embryos from callus of Sequoia sempervirens. Plant Cell Rep. 1988 Oct;7(6):445-8. doi: 10.1007/BF00269534. PMID: 24240266.
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Plant Cell Rep. 1988 Oct;7(6):445-8. doi: 10.1007/BF00269534.

Somatic embryos from callus of Sequoia sempervirens.

Plant cell reports

F Bourgkard, J M Favre

Affiliations

  1. Laboratoire de Biologie des Ligneux, JE C.N.R.S. 034613, Université de Nancy I, B.P. 239, F-54506, Vandoeuvre Cedex, France.

PMID: 24240266 DOI: 10.1007/BF00269534

Abstract

Compact calli with a potential for somatic embryogenesis were obtained from complete or split mature zygotic embryos or from cotyledons and hypocotyls of in vitro grown seedlings of Sequoia sempervirens. Somatic embryos which showed a typical bipolar structure, were formed together with adventitious buds. When placed on filter paper supports they developed into complete plantlets. Of the various combinations tested, culture medium adapted from Murashige and Skoog mineral solution complemented with 6-benzylaminopurine (2 μM), kinetin (2 μM) and 2,4-dichlorophenoxyacetic acid (2.5 μM) was established as the optimal for somatic embryo production.

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