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Di Fonzo N, Manzocchi L, Salamini F, et al. Purification and properties of an endospermic protein of maize associated with the Opaque-2 and Opaque-6 genes. Planta. 1986;167(4):587-94doi: 10.1007/BF00391237.
Di Fonzo, N., Manzocchi, L., Salamini, F., & Soave, C. (1986). Purification and properties of an endospermic protein of maize associated with the Opaque-2 and Opaque-6 genes. Planta, 167(4), 587-94. https://doi.org/10.1007/BF00391237
Di Fonzo, N, et al. "Purification and properties of an endospermic protein of maize associated with the Opaque-2 and Opaque-6 genes." Planta vol. 167,4 (1986): 587-94. doi: https://doi.org/10.1007/BF00391237
Di Fonzo N, Manzocchi L, Salamini F, Soave C. Purification and properties of an endospermic protein of maize associated with the Opaque-2 and Opaque-6 genes. Planta. 1986 Apr;167(4):587-94. doi: 10.1007/BF00391237. PMID: 24240377.
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Planta. 1986 Apr;167(4):587-94. doi: 10.1007/BF00391237.

Purification and properties of an endospermic protein of maize associated with the Opaque-2 and Opaque-6 genes.

Planta

N Di Fonzo, L Manzocchi, F Salamini, C Soave

Affiliations

  1. Sezione Maiscoltura, Istituto Sperimentale per la Cerealicoltura, Bergamo, Italy.

PMID: 24240377 DOI: 10.1007/BF00391237

Abstract

Maize endosperms accumulate during development a large amount of storage proteins (zeins). The rate of zein accumulation is under the control of several regulatory genes. Two of these, the opaque-2 and opaque-6 mutants, lower the zein level, thus improving the nutritional quality of maize meals. An endosperm protein of Mr 32 000 (b-32) appears to be correlated with the zein level. The b-32 protein is encoded by the opaque-6 gene which, in turn, is activated by opaque-2. We report the purification, amino-acid composition and peptide map of b-32 protein. Furthermore we demonstrate that the protein exists as a monomer likely located in the soluble cytoplasm. As a step towards the isolation of a complementary-DNA clone for b-32 protein, the purification of its corresponding mRNA is described.

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