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Harris N, Croy RR. The major albumin protein from pea (Pisum sativum L.) : Localisation by immunocytochemistry. Planta. 1985;165(4):522-6doi: 10.1007/BF00398098.
Harris, N., & Croy, R. R. (1985). The major albumin protein from pea (Pisum sativum L.) : Localisation by immunocytochemistry. Planta, 165(4), 522-6. https://doi.org/10.1007/BF00398098
Harris, N, and Croy, R R. "The major albumin protein from pea (Pisum sativum L.) : Localisation by immunocytochemistry." Planta vol. 165,4 (1985): 522-6. doi: https://doi.org/10.1007/BF00398098
Harris N, Croy RR. The major albumin protein from pea (Pisum sativum L.) : Localisation by immunocytochemistry. Planta. 1985 Sep;165(4):522-6. doi: 10.1007/BF00398098. PMID: 24241226.
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Planta. 1985 Sep;165(4):522-6. doi: 10.1007/BF00398098.

The major albumin protein from pea (Pisum sativum L.) : Localisation by immunocytochemistry.

Planta

N Harris, R R Croy

Affiliations

  1. Department of Botany, University of Durham, South Road, DH1 3LE, Durham, U.K..

PMID: 24241226 DOI: 10.1007/BF00398098

Abstract

The major albumin protein in storage parenchyma tissue of developing peas has been localised at an ultrastructural level by immunocytochemistry. Tissue was fixed in buffered aldehyde and embedded in LR White resin which was polymerised by addition of catalyst. Sections were labelled by the indirect method of absorption of Protein A-gold to specifically bound antibodies. This method gives high levels of specific labelling on sections which retain good ultrastructural preservation and have high contrast after conventional staining. The albumin is located throughout the cytoplasm although no labelling was found associated with the endoplasmic reticulum, Golgi apparatus, vacuoles-protein bodies or other organelles.

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