Display options
Cite
Oaks A, Stulen I, Jones K, et al. Enzymes of nitrogen assimilation in maize roots. Planta. 1980;148(5):477-84doi: 10.1007/BF00552663.
Oaks, A., Stulen, I., Jones, K., Winspear, M. J., Misra, S., & Boesel, I. L. (1980). Enzymes of nitrogen assimilation in maize roots. Planta, 148(5), 477-84. https://doi.org/10.1007/BF00552663
Oaks, A, et al. "Enzymes of nitrogen assimilation in maize roots." Planta vol. 148,5 (1980): 477-84. doi: https://doi.org/10.1007/BF00552663
Oaks A, Stulen I, Jones K, Winspear MJ, Misra S, Boesel IL. Enzymes of nitrogen assimilation in maize roots. Planta. 1980 Oct;148(5):477-84. doi: 10.1007/BF00552663. PMID: 24310191.
Copy Download .nbib Format: NLM
Share it on

Planta. 1980 Oct;148(5):477-84. doi: 10.1007/BF00552663.

Enzymes of nitrogen assimilation in maize roots.

Planta

A Oaks, I Stulen, K Jones, M J Winspear, S Misra, I L Boesel

Affiliations

  1. Biology Department, McMaster University, L8S 4K1, Hamilton, Ont., Canada.

PMID: 24310191 DOI: 10.1007/BF00552663

Abstract

The enzymes nitrate reductase (NR), glutamate dehydrogenase (GDH), glutamate synthase (GOGAT), glutamine synthetase (GS) and asparagine synthetase (AS) have been assayed in various regions along the seedling root ofZea mays L. In the intact attached root and calculated on a protein basis NR, GOGAT, and GS are found to have slightly higher specific activities in the apical 5 mm than in more mature regions of the root. GDH and AS, on the other hand, are much more active in extracts prepared from mature regions of the root than in the apical region. In excised root tips incubated in the presence of NH4 (+) and NO3 (-) there was a marked increase in GDH and AS, and a slight decrease in GOGAT and GS. Additions of NO3 (-) are required for NR activity but neither NO3 (-) nor NH4 (+) additions altered the activity levels of the other four enzymes. Additions of glucose to the medium inhibited the development of AS and GDH activities and resulted in higher activity levels of NR, GS and GOGAT. Glucose additions also enhanced the incorporation of acetate-(14)C and leucine-(14)C into protein. Additions of cycloheximide inhibit the development of NR, AS and GDH activities and also the incorporation of acetate-(14)C and leucine into protein.

References

  1. Planta. 1974 Dec;119(4):335-49 - PubMed
  2. Plant Physiol. 1976 Mar;57(3):339-43 - PubMed
  3. Plant Physiol. 1974 Oct;54(4):550-5 - PubMed
  4. Plant Physiol. 1977 Mar;59(3):391-4 - PubMed
  5. Planta. 1976 Jan;129(3):203-10 - PubMed
  6. Plant Physiol. 1975 Nov;56(5):634-9 - PubMed
  7. Planta. 1979 Jan;146(2):237-41 - PubMed
  8. Plant Physiol. 1976 Jun;57(6):881-5 - PubMed
  9. Plant Physiol. 1972 Dec;50(6):649-54 - PubMed
  10. Plant Physiol. 1979 May;63(5):793-5 - PubMed
  11. Plant Physiol. 1977 Nov;60(5):680-3 - PubMed
  12. Plant Physiol. 1979 Feb;63(2):232-6 - PubMed
  13. Plant Physiol. 1973 Sep;52(3):191-6 - PubMed
  14. Biochem Biophys Res Commun. 1974 Jun 4;58(3):639-46 - PubMed
  15. Arch Biochem Biophys. 1973 Nov;159(1):113-22 - PubMed
  16. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
  17. Plant Physiol. 1976 Oct;58(4):588-91 - PubMed
  18. Planta. 1979 Jan;145(3):287-92 - PubMed
  19. Plant Physiol. 1972 Feb;49(2):138-41 - PubMed

Publication Types