Display options
Cite
Marta Evans I, Gatehouse JA, Lycett GW, et al. Use of synthetic oligodeoxyribonucleotides and primed cDNA as probes for pea (Pisum sativum L.) RNA and genomic DNA sequences. Plant Mol Biol. 1984;3(2):73-81doi: 10.1007/BF00040031.
Marta Evans, I., Gatehouse, J. A., Lycett, G. W., & Boulter, D. (1984). Use of synthetic oligodeoxyribonucleotides and primed cDNA as probes for pea (Pisum sativum L.) RNA and genomic DNA sequences. Plant molecular biology, 3(2), 73-81. https://doi.org/10.1007/BF00040031
Marta Evans, I, et al. "Use of synthetic oligodeoxyribonucleotides and primed cDNA as probes for pea (Pisum sativum L.) RNA and genomic DNA sequences." Plant molecular biology vol. 3,2 (1984): 73-81. doi: https://doi.org/10.1007/BF00040031
Marta Evans I, Gatehouse JA, Lycett GW, Boulter D. Use of synthetic oligodeoxyribonucleotides and primed cDNA as probes for pea (Pisum sativum L.) RNA and genomic DNA sequences. Plant Mol Biol. 1984 Mar;3(2):73-81. doi: 10.1007/BF00040031. PMID: 24310302.
Copy Download .nbib Format: NLM
Share it on

Plant Mol Biol. 1984 Mar;3(2):73-81. doi: 10.1007/BF00040031.

Use of synthetic oligodeoxyribonucleotides and primed cDNA as probes for pea (Pisum sativum L.) RNA and genomic DNA sequences.

Plant molecular biology

I Marta Evans, J A Gatehouse, G W Lycett, D Boulter

Affiliations

  1. Department of Botany, University of Durham, South Road, DH1 3LE, Durham, U.K..

PMID: 24310302 DOI: 10.1007/BF00040031

Abstract

Two oligonucleotide sequences were synthesised by a solid-phase phosphotriester method. One of these sequences, A was a copy of part of a characterised cDNA clone encoding the basic subunit of legumin, a seed storage protein of Pisum sativum L. (garden pea); the other sequence B was predicted to be complementary to the 5' region of legumin mRNA on the basis of the amino acid sequence of legumin acidic subunits and most likely codon usage. Sequence A was shown to hybridise specifically to a legumin cDNA clone and to legumin mRNA. Sequence B did not hybridise specifically to legumin mRNA and was concluded not to be correctly complementary to legumin mRNA. Sequence A was used as a primer for cDNA synthesis using pea seed mRNA as a template. The cDNA so produced hybridised specifically to a legumin cDNA clone, to legumin mRNA, and to sequences encoding legumin in a restriction digest of pea genomic DNA. It is suggested that such oligonucleotide primed cDNAs may be of general value in probing eukaryotic genomic DNA.

References

  1. Nucleic Acids Res. 1982 Mar 11;10(5):1495-502 - PubMed
  2. Cell. 1978 Jul;14(3):673-80 - PubMed
  3. Nucleic Acids Res. 1982 Oct 25;10(20):6243-54 - PubMed
  4. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 - PubMed
  5. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 - PubMed
  6. Nucleic Acids Res. 1982 Dec 20;10(24):8191-210 - PubMed
  7. Nucleic Acids Res. 1979;6(6):2069-87 - PubMed
  8. Planta. 1979 Jan;144(5):455-62 - PubMed
  9. Methods Enzymol. 1980;65(1):499-560 - PubMed
  10. Nature. 1982 Feb 4;295(5848):380-4 - PubMed
  11. Biochim Biophys Acta. 1980 Nov 14;610(1):81-95 - PubMed
  12. Nature. 1981 Aug 20;292(5825):756-62 - PubMed
  13. J Biol Chem. 1981 Jan 25;256(2):1023-8 - PubMed
  14. Nucleic Acids Res. 1981 Feb 25;9(4):879-94 - PubMed

Publication Types