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Davis DG, Olson PA, Stolzenberg RL. Organogenesis in cell cultures of leafy spurge (Euphorbiaceae) accessions from Europe and North America. Plant Cell Rep. 1988;7(4):253-6doi: 10.1007/BF00272536.
Davis, D. G., Olson, P. A., & Stolzenberg, R. L. (1988). Organogenesis in cell cultures of leafy spurge (Euphorbiaceae) accessions from Europe and North America. Plant cell reports, 7(4), 253-6. https://doi.org/10.1007/BF00272536
Davis, D G, et al. "Organogenesis in cell cultures of leafy spurge (Euphorbiaceae) accessions from Europe and North America." Plant cell reports vol. 7,4 (1988): 253-6. doi: https://doi.org/10.1007/BF00272536
Davis DG, Olson PA, Stolzenberg RL. Organogenesis in cell cultures of leafy spurge (Euphorbiaceae) accessions from Europe and North America. Plant Cell Rep. 1988 Jun;7(4):253-6. doi: 10.1007/BF00272536. PMID: 24241760.
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Plant Cell Rep. 1988 Jun;7(4):253-6. doi: 10.1007/BF00272536.

Organogenesis in cell cultures of leafy spurge (Euphorbiaceae) accessions from Europe and North America.

Plant cell reports

D G Davis, P A Olson, R L Stolzenberg

Affiliations

  1. USDA/ARS Metabolism & Radiation Research Laboratory, State University Station, 58105, Fargo, ND, USA.

PMID: 24241760 DOI: 10.1007/BF00272536

Abstract

Plants were regenerated from leafy spurge (Euphorbia esula L.) cell suspensions obtained from stem callus. A North Dakota accession was highly regenerable, but two accessions from Oregon and Austria formed only a few plantlets. Organogenesis occurred in media without growth regulators, under fluorescent lights (30 to 90 μE m(-2) s(-1), 14 h photoperiod). Organogenesis was greatest in larger size clumps subcultured during maximum cell growth into media containing a reduced:oxidized nitrogen ratio of 33:67. Roots formed first and some clumps produced shoots. Organogenic suspension cultures also were initiated from hypocotyl and root segments of germinated seedlings, directly in liquid medium. Plantlets of the North Dakota accession formed in vitro adapted to greenhouse conditions. They were phenotypically similar to the parent plants.

References

  1. Plant Physiol. 1985 Dec;79(4):988-91 - PubMed

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