Display options
Cite
Nguyen J, Machal L, Vidal J, et al. Immunochemical studies on xanthine dehydrogenase of soybean root nodules : Ontogenic changes in the level of enzyme and immunocytochemical localization. Planta. 1986;167(2):190-5doi: 10.1007/BF00391414.
Nguyen, J., Machal, L., Vidal, J., Perrot-Rechenmann, C., & Gadal, P. (1986). Immunochemical studies on xanthine dehydrogenase of soybean root nodules : Ontogenic changes in the level of enzyme and immunocytochemical localization. Planta, 167(2), 190-5. https://doi.org/10.1007/BF00391414
Nguyen, J, et al. "Immunochemical studies on xanthine dehydrogenase of soybean root nodules : Ontogenic changes in the level of enzyme and immunocytochemical localization." Planta vol. 167,2 (1986): 190-5. doi: https://doi.org/10.1007/BF00391414
Nguyen J, Machal L, Vidal J, Perrot-Rechenmann C, Gadal P. Immunochemical studies on xanthine dehydrogenase of soybean root nodules : Ontogenic changes in the level of enzyme and immunocytochemical localization. Planta. 1986 Feb;167(2):190-5. doi: 10.1007/BF00391414. PMID: 24241850.
Copy Download .nbib Format: NLM
Share it on

Planta. 1986 Feb;167(2):190-5. doi: 10.1007/BF00391414.

Immunochemical studies on xanthine dehydrogenase of soybean root nodules : Ontogenic changes in the level of enzyme and immunocytochemical localization.

Planta

J Nguyen, L Machal, J Vidal, C Perrot-Rechenmann, P Gadal

Affiliations

  1. Institut de Physiologie Végétale, Centre National de la Recherche Scientifique, F-91190, Gif-sur-Yvette, France.

PMID: 24241850 DOI: 10.1007/BF00391414

Abstract

Xanthine dehydrogenase (XDH, EC 1.2.1.37) was purified from root nodules of soybean (Glycine max) and used to prepare a polyclonal rabbit antiserum. Monospecificity of this antiserum was ascertained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the immunoprecipate. During root nodule development of soybean, only one form of XDH was detected on an immunological basis. Titration of XDH by immunoelectrophoresis showed that a remarkable increase in the amount of XDH occurred between two and four weeks after inoculation, in parallel with the increase in enzyme activity. Localization of XDH by immunofluorescence indicated that the enzyme was present exclusively in uninfected cells where it appeared to be associated with discrete organellels.

References

  1. FEBS Lett. 1972 Nov 15;28(1):73-6 - PubMed
  2. Plant Physiol. 1979 Sep;64(3):411-6 - PubMed
  3. Prog Allergy. 1958;5:1-78 - PubMed
  4. Planta. 1982 Jun;155(1):45-51 - PubMed
  5. Arch Biochem Biophys. 1983 Apr 15;222(2):435-41 - PubMed
  6. Plant Physiol. 1978 Jul;62(1):134-8 - PubMed
  7. Biochim Biophys Acta. 1970 Nov 24;222(2):556-7 - PubMed
  8. Arch Biochem Biophys. 1982 Nov;219(1):39-46 - PubMed
  9. Plant Physiol. 1980 Jun;65(6):1203-6 - PubMed
  10. Plant Physiol. 1983 Apr;71(4):869-73 - PubMed
  11. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
  12. Science. 1981 Jun 19;212(4501):1394-6 - PubMed
  13. J Biol Chem. 1969 Aug 25;244(16):4406-12 - PubMed
  14. Anal Biochem. 1976 May 7;72:248-54 - PubMed
  15. Cell. 1980 May;20(1):153-63 - PubMed
  16. EMBO J. 1983;2(12):2333-9 - PubMed
  17. Anal Biochem. 1966 Apr;15(1):45-52 - PubMed
  18. Arch Biochem Biophys. 1983 Jul 15;224(2):429-41 - PubMed
  19. Plant Physiol. 1981 Jul;68(1):65-9 - PubMed
  20. Plant Physiol. 1968 Aug;43(8):1185-207 - PubMed
  21. Planta. 1982 Jun;155(1):24-30 - PubMed
  22. Plant Physiol. 1978 Oct;62(4):495-8 - PubMed
  23. Plant Physiol. 1985 Apr;77(4):1004-9 - PubMed

Publication Types