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Vincentz M, Gigot C. HMG-like protein in barley and corn nuclei. Plant Mol Biol. 1985;4(2):161-8doi: 10.1007/BF02418763.
Vincentz, M., & Gigot, C. (1985). HMG-like protein in barley and corn nuclei. Plant molecular biology, 4(2), 161-8. https://doi.org/10.1007/BF02418763
Vincentz, M, and Gigot, C. "HMG-like protein in barley and corn nuclei." Plant molecular biology vol. 4,2 (1985): 161-8. doi: https://doi.org/10.1007/BF02418763
Vincentz M, Gigot C. HMG-like protein in barley and corn nuclei. Plant Mol Biol. 1985 Mar;4(2):161-8. doi: 10.1007/BF02418763. PMID: 24310753.
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Plant Mol Biol. 1985 Mar;4(2):161-8. doi: 10.1007/BF02418763.

HMG-like protein in barley and corn nuclei.

Plant molecular biology

M Vincentz, C Gigot

Affiliations

  1. Laboratoire de Virologie, Institut de Biologie Moléculaire et Cellulaire, 15, rue Descartes, 67084, Strasbourg, France.

PMID: 24310753 DOI: 10.1007/BF02418763

Abstract

Chromosomal proteins have been isolated from barley (Hordeum vulgare) and corn (Zea mays) nuclei by extraction with 5% perchloric acid. In each plant, one protein was shown to belong to the HMG proteins. Their molecular weights are very close to that of HMG 14 from chicken erythrocytes, as shown by electrophoretic mobility in SDS polyacrylamide gels. In acetic acid-urea-Triton polyacrylamide gels they migrate between HMG 1,2 and HMG 14, from chicken erythrocytes. Their amino acid compositions are typical of HMG proteins, with equivalent high values of acidic and basic residues.Extraction of HMG's from purified barley chromatin fractions with 0.35 M NaCl considerably reduces histone H2 contamination and increases the yield of HMG up to 0.7% of the total histones. In this technique a second protein was extracted which is soluble in 2% Trichloroacetic acid and shows electrophoretic mobility analogous to those of HMG 14 and 17 from chicken erythrocytes. Whether or not these proteins are counterparts of the animal HMG's 1-2 or HMG's 14-17 is discussed.

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