Display options
Cite
Burgess SW, Wu JR, Swift K, et al. Determination of the rate of rapid lipid transfer induced by poly(ethylene glycol) using the SLM Fourier transform phase and modulation spectrofluorometer. J Fluoresc. 1991;1(2):105-12doi: 10.1007/BF00865206.
Burgess, S. W., Wu, J. R., Swift, K., & Lentz, B. R. (1991). Determination of the rate of rapid lipid transfer induced by poly(ethylene glycol) using the SLM Fourier transform phase and modulation spectrofluorometer. Journal of fluorescence, 1(2), 105-12. https://doi.org/10.1007/BF00865206
Burgess, S W, et al. "Determination of the rate of rapid lipid transfer induced by poly(ethylene glycol) using the SLM Fourier transform phase and modulation spectrofluorometer." Journal of fluorescence vol. 1,2 (1991): 105-12. doi: https://doi.org/10.1007/BF00865206
Burgess SW, Wu JR, Swift K, Lentz BR. Determination of the rate of rapid lipid transfer induced by poly(ethylene glycol) using the SLM Fourier transform phase and modulation spectrofluorometer. J Fluoresc. 1991 Jun;1(2):105-12. doi: 10.1007/BF00865206. PMID: 24242960.
Copy Download .nbib Format: NLM
Share it on

J Fluoresc. 1991 Jun;1(2):105-12. doi: 10.1007/BF00865206.

Determination of the rate of rapid lipid transfer induced by poly(ethylene glycol) using the SLM Fourier transform phase and modulation spectrofluorometer.

Journal of fluorescence

S W Burgess, J R Wu, K Swift, B R Lentz

Affiliations

  1. Department of Biochemistry & Biophysics, University of North Carolina, 27599-7260, Chapel Hill, North Carolina.

PMID: 24242960 DOI: 10.1007/BF00865206

Abstract

Rate constants were determined for the transfer of the fluorescent lipid probe 1-palmitoyl-2-[[2-[4-(6-phenyl-trans-1,3,5-hexatrienyl)phenyl]ethyl] oxy]carbonyl]-3-sn-phosphatidylcholine (DPHpPC) between large, unilamellar extrusion vesicles composed either of dipalmitoyl phosphatidylcholine (DPPC) or of DPPC mixed with a small amount (0.5 mol%) of lyso phosphatidylcholine (Lyso PC). Transfer of the lipid probe in the presence of varying concentrations of poly(ethylene glycol) (PEG) was monitored using the SLM 48000-MHF Multi-Harmonic Fourier Transform phase and modulation spectrofluorometer to collect multifrequency phase and modulation fluorescence data sets on a subsecond time scale. The unique ability of this instrument to yield accurate fluorescence lifetime data on this time scale allowed transfer to be detected in terms of a time-dependent change in the fluorescent lifetime distribution associated with the lipid-like DPHpPC probe. This probe demonstrates two short fluoresence decay times (ca. 1.1-1.4 and 4.3-4.8 ns) in a probe-rich environment but a single long lifetime (ca. 7 ns) in a probe-poor environment. A simple two-state model for initial lipid transfer was used to analyze the multifrequency data sets collected over a 4-s time frame to obtain the time rate of change of the concentrations of donor and acceptor probe populations following rapid mixing of vesicles with PEG. The ability to measure fluorescence lifetimes on this time scale has allowed us to show that the of rate of lipid transfer increased dramatically at 35% PEG in both fusing and nonfusing vesicle systems. These results are interpreted in terms of a distinct interbilayer structure associated with intimate bilayer contact induced by high and potentially fusogenic concentrations of PEG.

References

  1. Biophys J. 1989 Oct;56(4):723-33 - PubMed
  2. Biochemistry. 1987 Jun 16;26(12):3267-76 - PubMed
  3. Biochemistry. 1980 Dec 23;19(26):6050-7 - PubMed
  4. Biochemistry. 1990 Feb 13;29(6):1593-600 - PubMed
  5. Biochemistry. 1991 Jul 9;30(27):6780-7 - PubMed
  6. Biochemistry. 1987 Nov 17;26(23):7325-32 - PubMed
  7. Biochemistry. 1986 Mar 11;25(5):1021-6 - PubMed
  8. Biochemistry. 1976 Oct 5;15(20):4529-37 - PubMed
  9. Biochemistry. 1986 Oct 21;25(21):6678-88 - PubMed
  10. Biochemistry. 1985 Jun 18;24(13):3091-8 - PubMed
  11. Biochemistry. 1991 Apr 30;30(17):4193-200 - PubMed
  12. Science. 1989 Nov 17;246(4932):919-22 - PubMed
  13. Biochim Biophys Acta. 1985 Jan 10;812(1):55-65 - PubMed
  14. Biochim Biophys Acta. 1987 Oct 5;906(3):309-52 - PubMed

Publication Types