Display options
Cite
Moseley MA, Jorgenson JW, Shabanowitz J, et al. Optimization of capillary zone electrophoresis/electrospray ionization parameters for the mass spectrometry and tandem mass spectrometry analysis of peptides. J Am Soc Mass Spectrom. 1992;3(4):289-300doi: 10.1016/1044-0305(92)87056-5.
Moseley, M. A., Jorgenson, J. W., Shabanowitz, J., Hunt, D. F., & Tomer, K. B. (1992). Optimization of capillary zone electrophoresis/electrospray ionization parameters for the mass spectrometry and tandem mass spectrometry analysis of peptides. Journal of the American Society for Mass Spectrometry, 3(4), 289-300. https://doi.org/10.1016/1044-0305(92)87056-5
Moseley, M A, et al. "Optimization of capillary zone electrophoresis/electrospray ionization parameters for the mass spectrometry and tandem mass spectrometry analysis of peptides." Journal of the American Society for Mass Spectrometry vol. 3,4 (1992): 289-300. doi: https://doi.org/10.1016/1044-0305(92)87056-5
Moseley MA, Jorgenson JW, Shabanowitz J, Hunt DF, Tomer KB. Optimization of capillary zone electrophoresis/electrospray ionization parameters for the mass spectrometry and tandem mass spectrometry analysis of peptides. J Am Soc Mass Spectrom. 1992 May;3(4):289-300. doi: 10.1016/1044-0305(92)87056-5. PMID: 24243039.
Copy Download .nbib Format: NLM
Share it on

J Am Soc Mass Spectrom. 1992 May;3(4):289-300. doi: 10.1016/1044-0305(92)87056-5.

Optimization of capillary zone electrophoresis/electrospray ionization parameters for the mass spectrometry and tandem mass spectrometry analysis of peptides.

Journal of the American Society for Mass Spectrometry

M A Moseley, J W Jorgenson, J Shabanowitz, D F Hunt, K B Tomer

Affiliations

  1. Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina, USA.

PMID: 24243039 DOI: 10.1016/1044-0305(92)87056-5

Abstract

The solution chemistry conditions necessary for optimum analysis of peptides by capillary zone electrophoresis (CZE)/electrospray ionization mass spectrometry and CZE electrospray ionization tandem mass spectrometry have been studied. To maximize the signal-to-noise ratio of the spectra it was found necessary to use acidic CZE buffers of low ionic strength. This not only increases the total ion current, but it also serves to fully protonate the peptides, minimizing the distribution of ion current across the ensemble of possible charge states.The use of acidic buffers protonates the peptides, which is advantageous for mass spectrometry and tandem mass spectrometry analysis, but is problematic with CZE when bare fused silica CZE columns are used. These conditions produce positively charged peptides, and negatively charged silanol moieties on the column wall, inducing adsorption of the positively charged peptides, thus causing zone broadening and a loss in separation efficiency. This problem was circumvented by the preparation of chemically modified CZE columns, which, when used with acidic CZE buffers, will have a positively charged inner column wall. The electrostatic repulsion between the positively charged peptides and the positively charged CZE column wall minimizes adsorption problems and facilitates high efficiency separations. Full-scan mass spectra were acquired from injections of as little as 160 fmols of test peptides, with CZE separation efficiencies of up to 250,000 theoretical plates.

References

  1. J Chromatogr. 1989 Oct 20;480:197-209 - PubMed
  2. J Chromatogr. 1988 Dec 23;458:313-21 - PubMed
  3. Anal Chem. 1990 May 1;62(9):882-99 - PubMed
  4. Anal Chem. 1986 Jun;58(7):743A-760A - PubMed
  5. J Chromatogr. 1990 Sep 7;516(1):69-78 - PubMed
  6. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6233-7 - PubMed
  7. Rapid Commun Mass Spectrom. 1989 Mar;3(3):87-93 - PubMed
  8. Anal Chem. 1985 Mar;57(3):675-9 - PubMed
  9. Anal Chem. 1991 Jan 15;63(2):109-14 - PubMed
  10. Anal Chem. 1988 Nov 1;60(21):2322-8 - PubMed
  11. J Chromatogr. 1989 Oct 20;480:301-10 - PubMed
  12. Science. 1989 Oct 6;246(4926):64-71 - PubMed
  13. Biopolymers. 1971;10(5):821-6 - PubMed
  14. Science. 1983 Oct 21;222(4621):266-72 - PubMed
  15. Anal Chem. 1990 Nov 15;62(22):2478-83 - PubMed
  16. J Am Soc Mass Spectrom. 1990 Feb;1(1):53-65 - PubMed
  17. Proc Natl Acad Sci U S A. 1987 Feb;84(3):620-3 - PubMed
  18. J Chromatogr. 1988 Apr 1;438(1):23-34 - PubMed

Publication Types