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Hogeland KE, Arbogast B, Deinzer ML. Liquid secondary ion mass spectrometry analysis of permethylated, n-hexylamine derivatized oligosaccharides. Application to baculovirus expressed mouse interleukin-3. J Am Soc Mass Spectrom. 1992;3(4):345-52doi: 10.1016/1044-0305(92)87062-4.
Hogeland, K. E., Arbogast, B., & Deinzer, M. L. (1992). Liquid secondary ion mass spectrometry analysis of permethylated, n-hexylamine derivatized oligosaccharides. Application to baculovirus expressed mouse interleukin-3. Journal of the American Society for Mass Spectrometry, 3(4), 345-52. https://doi.org/10.1016/1044-0305(92)87062-4
Hogeland, K E, et al. "Liquid secondary ion mass spectrometry analysis of permethylated, n-hexylamine derivatized oligosaccharides. Application to baculovirus expressed mouse interleukin-3." Journal of the American Society for Mass Spectrometry vol. 3,4 (1992): 345-52. doi: https://doi.org/10.1016/1044-0305(92)87062-4
Hogeland KE, Arbogast B, Deinzer ML. Liquid secondary ion mass spectrometry analysis of permethylated, n-hexylamine derivatized oligosaccharides. Application to baculovirus expressed mouse interleukin-3. J Am Soc Mass Spectrom. 1992 May;3(4):345-52. doi: 10.1016/1044-0305(92)87062-4. PMID: 24243045.
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J Am Soc Mass Spectrom. 1992 May;3(4):345-52. doi: 10.1016/1044-0305(92)87062-4.

Liquid secondary ion mass spectrometry analysis of permethylated, n-hexylamine derivatized oligosaccharides. Application to baculovirus expressed mouse interleukin-3.

Journal of the American Society for Mass Spectrometry

K E Hogeland, B Arbogast, M L Deinzer

Affiliations

  1. Department of Chemistry, Oregon State University, Corvallis, Oregon, USA.

PMID: 24243045 DOI: 10.1016/1044-0305(92)87062-4

Abstract

Reductive amination with n-hexylamine followed by permethylation was used as a procedure for the liquid secondary ion mass spectrometry (LSIMS) analysis of Asn-linked oligosaccharides. Initial experiments with this procedure were performed on maltoheptaose. These experiments show that exhaustive methylation at the newly formed secondary nitrogen forms a quaternary ammonium salt. When this is subjected to positive ion LSIMS, an abundant M(+) ion is observed. This procedure was applied to the Asn-linked oligosaccharides released from human transferrin and ribonuclease-B. The reductively aminated, permethylated mixture of oligosaccharides from ribonuclease-B afforded a positive ion LSI mass spectrum in which M(+) ions for Mans5-9GlcNAc2 could be assigned. The positive ion LSI mass spectrum obtained from the mixture of oligosaccharides isolated from human transferrin showed M(+) ions that could be assigned to both monosialylated and disialylated biantennary complex type oligosaccharides. Reductive amination followed by permethylation of the Asn-linked oligosaccharides isolated from baculovirus expressed mouse interleukin-3 produced in Bombyx mori gave a positive ion LSI mass spectrum in which the oligosaccharides could be assigned the monosaccharide composition Man2-4[Fuc]GlcNAc2 and Man2GlcNAc2. These are believed to be dimannose, trimannose, and tetramannose chitobiose core oligosaccharides, three of which are fucosylated.

References

  1. Anal Biochem. 1991 May 15;195(1):1-13 - PubMed
  2. Anal Biochem. 1984 Sep;141(2):366-81 - PubMed
  3. Biochimie. 1988 Nov;70(11):1459-69 - PubMed
  4. Anal Biochem. 1987 May 1;162(2):485-92 - PubMed
  5. J Biochem. 1980 Jul;88(1):51-8 - PubMed
  6. J Chromatogr. 1990 Jan 19;499:205-20 - PubMed
  7. Carbohydr Res. 1989 Jun 1;188:1-7 - PubMed
  8. Anal Biochem. 1991 Jan;192(1):181-92 - PubMed
  9. Proc Natl Acad Sci U S A. 1987 Mar;84(5):1369-73 - PubMed
  10. Proc Natl Acad Sci U S A. 1988 May;85(10):3289-93 - PubMed
  11. Annu Rev Biochem. 1988;57:785-838 - PubMed
  12. Biochemistry. 1985 Aug 13;24(17):4665-71 - PubMed
  13. Biomed Environ Mass Spectrom. 1988 Oct;16(1-12):19-24 - PubMed
  14. Anal Biochem. 1988 Mar;169(2):337-49 - PubMed
  15. J Biol Chem. 1988 Oct 25;263(30):15713-9 - PubMed
  16. Gene. 1987;58(2-3):273-81 - PubMed
  17. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1070-4 - PubMed
  18. Biochim Biophys Acta. 1985;780(2):119-50 - PubMed
  19. J Biochem. 1979 Jan;85(1):217-20 - PubMed
  20. Biochem Biophys Res Commun. 1985 Oct 15;132(1):1-7 - PubMed
  21. J Biol Chem. 1986 Jan 5;261(1):172-7 - PubMed
  22. Anal Biochem. 1988 Nov 1;174(2):459-70 - PubMed
  23. Arch Biochem Biophys. 1987 Jul;256(1):194-201 - PubMed
  24. FEBS Lett. 1975 Feb 15;50(3):296-9 - PubMed
  25. Anal Biochem. 1990 Nov 15;191(1):174-82 - PubMed
  26. Adv Carbohydr Chem Biochem. 1987;45:19-72 - PubMed
  27. J Chromatogr. 1991 Jan 2;562(1-2):403-19 - PubMed

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