Display options
Cite
Salih G, Wiklund R, Tyystjärvi T, et al. Constructed deletions in lumen-exposed regions of the D1 protein in the cyanobacterium Synechocystis 6803: Effects on D1 insertion and accumulation in the thylakoid membrane, and on Photosystem II assembly. Photosynth Res. 1996;49(2):131-40doi: 10.1007/BF00117663.
Salih, G., Wiklund, R., Tyystjärvi, T., Mäenpää, P., Gerez, C., & Jansson, C. (1996). Constructed deletions in lumen-exposed regions of the D1 protein in the cyanobacterium Synechocystis 6803: Effects on D1 insertion and accumulation in the thylakoid membrane, and on Photosystem II assembly. Photosynthesis research, 49(2), 131-40. https://doi.org/10.1007/BF00117663
Salih, G, et al. "Constructed deletions in lumen-exposed regions of the D1 protein in the cyanobacterium Synechocystis 6803: Effects on D1 insertion and accumulation in the thylakoid membrane, and on Photosystem II assembly." Photosynthesis research vol. 49,2 (1996): 131-40. doi: https://doi.org/10.1007/BF00117663
Salih G, Wiklund R, Tyystjärvi T, Mäenpää P, Gerez C, Jansson C. Constructed deletions in lumen-exposed regions of the D1 protein in the cyanobacterium Synechocystis 6803: Effects on D1 insertion and accumulation in the thylakoid membrane, and on Photosystem II assembly. Photosynth Res. 1996 Aug;49(2):131-40. doi: 10.1007/BF00117663. PMID: 24271610.
Copy Download .nbib Format: NLM
Share it on

Photosynth Res. 1996 Aug;49(2):131-40. doi: 10.1007/BF00117663.

Constructed deletions in lumen-exposed regions of the D1 protein in the cyanobacterium Synechocystis 6803: Effects on D1 insertion and accumulation in the thylakoid membrane, and on Photosystem II assembly.

Photosynthesis research

G Salih, R Wiklund, T Tyystjärvi, P Mäenpää, C Gerez, C Jansson

Affiliations

  1. Department of Biochemistry, The Arrhenius Laboratories, Stockholm University, S-10691, Stockholm, Sweden.

PMID: 24271610 DOI: 10.1007/BF00117663

Abstract

Modified forms of the D1 protein with deletions in lumen-exposed regions, were constructed in the cyanobacterium Synechocystis 6803 using site-directed mutagenesis. Integration and stability of the mutated D1 proteins in the thylakoid membrane were studied by immunoblot and pulse-chase analyses. It was found that in Δ(N325-E333), the D1 protein with a deletion in the C-terminal tail, could insert in the thylakoids to normal amounts but its stability in the membrane was dramatically reduced. Insertion of D1 in Δ(V58-D61) or Δ(D103-G109);G110R, with deletions in the A-B loop, was severely obstructed, For Δ(P350-T354), with a deletion in the processed region of the C-terminus of D1, no phenotypic effects were observed. The effects of failed D1 insertion or accumulation on Photosystem II assembly was monitored by immunoblot analysis. The conclusions from these experiments are that the extrinsic 33 kDa protein, CP43, and the β subunit of cytochrome b559 accumulate in the thylakoid membrane independently of the D1 protein, and that accumulation of the D2 protein and CP47 requires insertion but not necessarily accumulation of the D1 protein.

References

  1. Biochemistry. 1992 Nov 10;31(44):10859-71 - PubMed
  2. Anal Biochem. 1976 May 7;72:248-54 - PubMed
  3. Biochemistry. 1995 May 2;34(17):5859-82 - PubMed
  4. EMBO J. 1994 May 15;13(10):2267-72 - PubMed
  5. Eur J Biochem. 1986 Aug 1;158(3):477-82 - PubMed
  6. Cell. 1989 Jan 27;56(2):241-6 - PubMed
  7. Proc Natl Acad Sci U S A. 1990 Feb;87(4):1451-5 - PubMed
  8. Nature. 1985 Dec 19-1986 Jan 1;318(6047):618-24 - PubMed
  9. Plant Mol Biol. 1990 Jun;14(6):1051-4 - PubMed
  10. Biochemistry. 1992 Jan 28;31(3):942-8 - PubMed
  11. Plant Mol Biol. 1991 May;16(5):891-7 - PubMed
  12. Plant Mol Biol. 1993 Apr;22(1):1-12 - PubMed
  13. Plant Mol Biol. 1994 Jun;25(3):517-26 - PubMed
  14. Mol Microbiol. 1992 Apr;6(7):947-56 - PubMed
  15. Plant Cell. 1991 Apr;3(4):383-95 - PubMed
  16. Mol Gen Genet. 1993 Apr;238(1-2):161-8 - PubMed
  17. Z Naturforsch C J Biosci. 1991 Sep-Oct;46(9-10):765-76 - PubMed
  18. Gene. 1984 Sep;29(3):303-13 - PubMed
  19. Nature. 1989 Jan 26;337(6205):382-5 - PubMed
  20. Gene. 1988 Aug 15;68(1):119-38 - PubMed
  21. Plant Mol Biol. 1989 Dec;13(6):693-700 - PubMed
  22. Plant Physiol. 1987 Dec;85(4):1021-5 - PubMed
  23. Biochemistry. 1995 May 2;34(17):5839-58 - PubMed
  24. EMBO J. 1990 Jul;9(7):2051-9 - PubMed
  25. Biochim Biophys Acta. 1992 Oct 16;1102(3):269-352 - PubMed
  26. J Bacteriol. 1991 Dec;173(23):7525-33 - PubMed
  27. FEBS Lett. 1988 Nov 21;240(1-2):6-8 - PubMed
  28. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 - PubMed
  29. EMBO J. 1986 Jun;5(6):1149-58 - PubMed
  30. Biochemistry. 1992 Jul 28;31(29):6660-72 - PubMed
  31. Arch Biochem Biophys. 1992 Jun;295(2):340-7 - PubMed

Publication Types