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Possani LD, Bayón A, Tapia R. Synthesis of affinity chromatography resins for the purification of brain glutamate decarboxylase. Neurochem Res. 1977;2(1):51-7doi: 10.1007/BF00966021.
Possani, L. D., Bayón, A., & Tapia, R. (1977). Synthesis of affinity chromatography resins for the purification of brain glutamate decarboxylase. Neurochemical research, 2(1), 51-7. https://doi.org/10.1007/BF00966021
Possani, L D, et al. "Synthesis of affinity chromatography resins for the purification of brain glutamate decarboxylase." Neurochemical research vol. 2,1 (1977): 51-7. doi: https://doi.org/10.1007/BF00966021
Possani LD, Bayón A, Tapia R. Synthesis of affinity chromatography resins for the purification of brain glutamate decarboxylase. Neurochem Res. 1977 Feb;2(1):51-7. doi: 10.1007/BF00966021. PMID: 24271849.
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Neurochem Res. 1977 Feb;2(1):51-7. doi: 10.1007/BF00966021.

Synthesis of affinity chromatography resins for the purification of brain glutamate decarboxylase.

Neurochemical research

L D Possani, A Bayón, R Tapia

Affiliations

  1. Departamento de Biología Experimental, Instituto de Biología, Universidad Nacional Autónoma de México, Apartado Postal 70-600, México 20, D. F., México.

PMID: 24271849 DOI: 10.1007/BF00966021

Abstract

In the present paper we describe the synthesis of Sepharose-boundN-(5'-phosphopyridoxyl)-amino-oxyacetic acid,N-(5'-phosphopyridoxyl)-canaline, andN-(5'-phosphopyridoxyl)-α,γ-diaminobutyric acid (Seph-DAB-PLP), designed for binding specifically brain glutamate decarboxylase (GAD). The three Sepharose-N-(phosphopyridoxyl)-amino acids were capable of binding GAD from a mouse brain soluble preparation. The enzyme can be purified up to 25-fold in one step by affinity chromatography on Seph-DAB-PLP.

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