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Hui KS, Davis BA, Boulton AA. Analysis of copper in brain by the mass-spectrometric integrated-ioncurrent procedure. Neurochem Res. 1977;2(5):495-506doi: 10.1007/BF00966010.
Hui, K. S., Davis, B. A., & Boulton, A. A. (1977). Analysis of copper in brain by the mass-spectrometric integrated-ioncurrent procedure. Neurochemical research, 2(5), 495-506. https://doi.org/10.1007/BF00966010
Hui, K S, et al. "Analysis of copper in brain by the mass-spectrometric integrated-ioncurrent procedure." Neurochemical research vol. 2,5 (1977): 495-506. doi: https://doi.org/10.1007/BF00966010
Hui KS, Davis BA, Boulton AA. Analysis of copper in brain by the mass-spectrometric integrated-ioncurrent procedure. Neurochem Res. 1977 Oct;2(5):495-506. doi: 10.1007/BF00966010. PMID: 24272241.
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Neurochem Res. 1977 Oct;2(5):495-506. doi: 10.1007/BF00966010.

Analysis of copper in brain by the mass-spectrometric integrated-ioncurrent procedure.

Neurochemical research

K S Hui, B A Davis, A A Boulton

Affiliations

  1. Psychiatric Research Division, University Hospital, S7N 0W8, Saskatoon, Saskatchewan, Canada.

PMID: 24272241 DOI: 10.1007/BF00966010

Abstract

Through use of the high-resolution double-focusing mass spectrometer, copper has been identified in various regions of the mouse, rat, guinea pig, rabbit, and human brain. The procedure depends on converting the copper (in ashed tissue) to its chloride salt, followed by derivatization with tetraphenylporphyrin (TPP) to yield a TPP chelate. After chromatographic separation, this chelate is assessed in the mass spectrometer by the integrated-ion-current procedure. Deuterated metal TPP chelates and the rare stable isotope(65)Cu were used as internal standards. Whole brain values obtained were as follows: mouse, 6.67±0.16 (mean±SEM) μg/g wet weight of tissue; rat, 1.06±0.05; guinea pig, 5.40±0.63; and rabbit, 7.52±0.76. In the rat, the cerebellum contained the highest concentration (1.25 μg/g), and the striatum the lowest (0.70 μg/g). In the human brain, the cortex (gray) and the striatum were relatively the highest copper-containing regions, with the cerebellum (white) being the lowest.

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