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Galli CL, Commissiong JW, Karoum F, et al. Measurement of phenylalanine hydroxylating activity in vivo : (18)O-tyrosine in plasma after exposing rats to(18)O 2. Neurochem Res. 1977;2(6):657-69doi: 10.1007/BF00963778.
Galli, C. L., Commissiong, J. W., Karoum, F., Neff, N. H., & Costa, E. (1977). Measurement of phenylalanine hydroxylating activity in vivo : (18)O-tyrosine in plasma after exposing rats to(18)O 2. Neurochemical research, 2(6), 657-69. https://doi.org/10.1007/BF00963778
Galli, C L, et al. "Measurement of phenylalanine hydroxylating activity in vivo : (18)O-tyrosine in plasma after exposing rats to(18)O 2." Neurochemical research vol. 2,6 (1977): 657-69. doi: https://doi.org/10.1007/BF00963778
Galli CL, Commissiong JW, Karoum F, Neff NH, Costa E. Measurement of phenylalanine hydroxylating activity in vivo : (18)O-tyrosine in plasma after exposing rats to(18)O 2. Neurochem Res. 1977 Dec;2(6):657-69. doi: 10.1007/BF00963778. PMID: 24272324.
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Neurochem Res. 1977 Dec;2(6):657-69. doi: 10.1007/BF00963778.

Measurement of phenylalanine hydroxylating activity in vivo : (18)O-tyrosine in plasma after exposing rats to(18)O 2.

Neurochemical research

C L Galli, J W Commissiong, F Karoum, N H Neff, E Costa

Affiliations

  1. Laboratory of Preclinical Pharmacology National Institute of Mental Health, Saint Elizabeths Hospital, 20032, Washington, D.C..

PMID: 24272324 DOI: 10.1007/BF00963778

Abstract

A gas chromatographic-mass spectrometric method was developed for analyzing phenylalanine and tyrosine (Tyr) in plasma and brain. With this procedure, we were able to show that(18)O2 is incorporated into Tyr in vivo and that the presence of(18)O-Tyr in plasma is a relative measure of phenylalanine hydroxylating activity. Treatment withp-chlorophenylalanine decreases the(18)O incorporation into Tyr. Because of the simplicity of the procedure and its easy adaptability to human studies, the incorporation of(18)O2 into endogenous constituents might serve as a useful diagnostic procedure for some metabolic disorders, such as phenylketonuria. These results also indicate that labeling with(18)O cannot be used to measure the turnover rate of brain catecholamines as previously proposed.

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