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Rezaian MA, Skene KG, Ellis JG. Anti-sense RNAs of cucumber mosaic virus in transgenic plants assessed for control of the virus. Plant Mol Biol. 1988;11(4):463-71doi: 10.1007/BF00039027.
Rezaian, M. A., Skene, K. G., & Ellis, J. G. (1988). Anti-sense RNAs of cucumber mosaic virus in transgenic plants assessed for control of the virus. Plant molecular biology, 11(4), 463-71. https://doi.org/10.1007/BF00039027
Rezaian, M A, et al. "Anti-sense RNAs of cucumber mosaic virus in transgenic plants assessed for control of the virus." Plant molecular biology vol. 11,4 (1988): 463-71. doi: https://doi.org/10.1007/BF00039027
Rezaian MA, Skene KG, Ellis JG. Anti-sense RNAs of cucumber mosaic virus in transgenic plants assessed for control of the virus. Plant Mol Biol. 1988 Jul;11(4):463-71. doi: 10.1007/BF00039027. PMID: 24272403.
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Plant Mol Biol. 1988 Jul;11(4):463-71. doi: 10.1007/BF00039027.

Anti-sense RNAs of cucumber mosaic virus in transgenic plants assessed for control of the virus.

Plant molecular biology

M A Rezaian, K G Skene, J G Ellis

Affiliations

  1. CSIRO Division of Horticulture, P.O. Box 350, 5001, Adelaide, S.A., Australia.

PMID: 24272403 DOI: 10.1007/BF00039027

Abstract

Three synthetic genes for the production of anti-sense RNA to different regions of the cucumber mosaic virus (CMV) genome were constructed using virus-derived double-stranded cDNA coupled to a promoter sequence from cauliflower mosaic virus. The genes were used to transform tobacco plants by a Ti plasmid vector. Transgenic plants obtained with the three constructs produced anti-sense RNA at different levels. Plants expressing each of the three anti-sense RNAs were inoculated with CMV and their sensitivity to the virus infection was compared with the non-transformed plants. Only one plant line which expressed relatively low levels of one of the anti-sense RNAs showed resistance to CMV but other plants expressing the same or the other two antisense RNAs had similar sensitivity to CMV infection as the non-transformed plants.

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