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Hancu G, Simon B, Rusu A, et al. Principles of micellar electrokinetic capillary chromatography applied in pharmaceutical analysis. Adv Pharm Bull. 2013;3(1):1-8doi: 10.5681/apb.2013.001.
Hancu, G., Simon, B., Rusu, A., Mircia, E., & Gyéresi, A. (2013). Principles of micellar electrokinetic capillary chromatography applied in pharmaceutical analysis. Advanced pharmaceutical bulletin, 3(1), 1-8. https://doi.org/10.5681/apb.2013.001
Hancu, Gabriel, et al. "Principles of micellar electrokinetic capillary chromatography applied in pharmaceutical analysis." Advanced pharmaceutical bulletin vol. 3,1 (2013): 1-8. doi: https://doi.org/10.5681/apb.2013.001
Hancu G, Simon B, Rusu A, Mircia E, Gyéresi A. Principles of micellar electrokinetic capillary chromatography applied in pharmaceutical analysis. Adv Pharm Bull. 2013;3(1):1-8. doi: 10.5681/apb.2013.001. Epub 2013 Feb 07. PMID: 24312804; PMCID: PMC3846027.
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Adv Pharm Bull. 2013;3(1):1-8. doi: 10.5681/apb.2013.001. Epub 2013 Feb 07.

Principles of micellar electrokinetic capillary chromatography applied in pharmaceutical analysis.

Advanced pharmaceutical bulletin

Gabriel Hancu, Brigitta Simon, Aura Rusu, Eleonora Mircia, Arpád Gyéresi

Affiliations

  1. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Medicine and Pharmacy, Târgu Mure?, Romania.

PMID: 24312804 PMCID: PMC3846027 DOI: 10.5681/apb.2013.001

Abstract

Since its introduction capillary electrophoresis has shown great potential in areas where electrophoretic techniques have rarely been used before, including here the analysis of pharmaceutical substances. The large majority of pharmaceutical substances are neutral from electrophoretic point of view, consequently separations by the classic capillary zone electrophoresis; where separation is based on the differences between the own electrophoretic mobilities of the analytes; are hard to achieve. Micellar electrokinetic capillary chromatography, a hybrid method that combines chromatographic and electrophoretic separation principles, extends the applicability of capillary electrophoretic methods to neutral analytes. In micellar electrokinetic capillary chromatography, surfactants are added to the buffer solution in concentration above their critical micellar concentrations, consequently micelles are formed; micelles that undergo electrophoretic migration like any other charged particle. The separation is based on the differential partitioning of an analyte between the two-phase system: the mobile aqueous phase and micellar pseudostationary phase. The present paper aims to summarize the basic aspects regarding separation principles and practical applications of micellar electrokinetic capillary chromatography, with particular attention to those relevant in pharmaceutical analysis.

Keywords: Capillary chromatography; Capillary electrophoresis; Micellar electrokinetic; Pharmaceutical analysis

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