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Mohammadian J, Mansoori-Derakhshan S, Mohammadian M, et al. Construction of Yeast Recombinant Expression Vector Containing Human Epidermal Growth Factor (hEGF). Adv Pharm Bull. 2013;3(2):473-6doi: 10.5681/apb.2013.079.
Mohammadian, J., Mansoori-Derakhshan, S., Mohammadian, M., & Shekari-Khaniani, M. (2013). Construction of Yeast Recombinant Expression Vector Containing Human Epidermal Growth Factor (hEGF). Advanced pharmaceutical bulletin, 3(2), 473-6. https://doi.org/10.5681/apb.2013.079
Mohammadian, Jamal, et al. "Construction of Yeast Recombinant Expression Vector Containing Human Epidermal Growth Factor (hEGF)." Advanced pharmaceutical bulletin vol. 3,2 (2013): 473-6. doi: https://doi.org/10.5681/apb.2013.079
Mohammadian J, Mansoori-Derakhshan S, Mohammadian M, Shekari-Khaniani M. Construction of Yeast Recombinant Expression Vector Containing Human Epidermal Growth Factor (hEGF). Adv Pharm Bull. 2013;3(2):473-6. doi: 10.5681/apb.2013.079. Epub 2013 Aug 20. PMID: 24312882; PMCID: PMC3848235.
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Adv Pharm Bull. 2013;3(2):473-6. doi: 10.5681/apb.2013.079. Epub 2013 Aug 20.

Construction of Yeast Recombinant Expression Vector Containing Human Epidermal Growth Factor (hEGF).

Advanced pharmaceutical bulletin

Jamal Mohammadian, Sima Mansoori-Derakhshan, Masood Mohammadian, Mahmoud Shekari-Khaniani

Affiliations

  1. Department of Clinical Biochemistry, Division of Medical Biotechnology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

PMID: 24312882 PMCID: PMC3848235 DOI: 10.5681/apb.2013.079

Abstract

PURPOSE: The objective of this study was construction of recombinant hEGF-pPIC9 which may be used for expression of recombinant hEGF in following studies.

METHODS: EGF cDNA was purchased from Genecopoeia Company and used for PCR amplification. Prior to ligation, the PCR product and pPIC9 vector was digested with EcoRI and XhoI and ligated in pPIC9 vector and subjected to colony PCR screening and sequencing analysis.

RESULTS: PCR amplification of EGF cDNA using recombinant hEGF-pPIC9 vector as template was concluded in amplification of 197bp fragment. Construction of recombinant hEGF-pPIC9 of EGf gene was verified by PCR and sequencing.

CONCLUSION: Construction of Recombinant hEGF-pPIC9 was the primary stage for production and expression of EFG in the future study.

Keywords: Cloning; Epidermal Growth Factor; Expression; Polymerase Chain Reaction; Sequencing; pPIC9

References

  1. FEMS Microbiol Rev. 1990 Dec;7(3-4):279-83 - PubMed
  2. Gastroenterology Res. 2010 Jun;3(3):125-133 - PubMed
  3. Proteomics. 2007 May;7(9):1434-45 - PubMed
  4. Yeast. 1995 Nov;11(14):1331-44 - PubMed
  5. J Biol Chem. 1995 Nov 17;270(46):27954-60 - PubMed
  6. Biomaterials. 2006 Jun;27(18):3451-8 - PubMed
  7. Curr Opin Biotechnol. 1999 Oct;10(5):411-21 - PubMed
  8. Microsc Res Tech. 2001 Jun 1;53(5):360-71 - PubMed
  9. Life Sci. 2006 Jan 2;78(6):649-54 - PubMed
  10. J Biotechnol. 2005 Mar 30;116(3):251-60 - PubMed
  11. Protein Expr Purif. 1995 Dec;6(6):813-20 - PubMed
  12. Endocr Rev. 1990 Aug;11(3):418-42 - PubMed
  13. Science. 2003 Aug 29;301(5637):1244-6 - PubMed
  14. Annu Rev Physiol. 1989;51:67-80 - PubMed
  15. Mol Cell Biol. 1989 Jul;9(7):2771-8 - PubMed
  16. Curr Opin Biotechnol. 2002 Aug;13(4):329-32 - PubMed
  17. Protein Sci. 1998 Jun;7(6):1415-22 - PubMed
  18. Eur J Biochem. 1999 Feb;260(1):200-7 - PubMed
  19. Nucleic Acids Res. 1986 Nov 11;14(21):8427-46 - PubMed

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