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Keyhanmanesh R, Bagban H, Nazemiyeh H, et al. The Relaxant Effects of Different Methanolic Fractions of Nigella sativa on Guinea Pig Tracheal Chains. Iran J Basic Med Sci. 2013;16(2):123-8
Keyhanmanesh, R., Bagban, H., Nazemiyeh, H., Mirzaei Bavil, F., Alipour, M. R., & Ahmady, M. (2013). The Relaxant Effects of Different Methanolic Fractions of Nigella sativa on Guinea Pig Tracheal Chains. Iranian journal of basic medical sciences, 16(2), 123-8.
Keyhanmanesh, Rana, et al. "The Relaxant Effects of Different Methanolic Fractions of Nigella sativa on Guinea Pig Tracheal Chains." Iranian journal of basic medical sciences vol. 16,2 (2013): 123-8.
Keyhanmanesh R, Bagban H, Nazemiyeh H, Mirzaei Bavil F, Alipour MR, Ahmady M. The Relaxant Effects of Different Methanolic Fractions of Nigella sativa on Guinea Pig Tracheal Chains. Iran J Basic Med Sci. 2013 Feb;16(2):123-8. PMID: 24298378; PMCID: PMC3843853.
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Iran J Basic Med Sci. 2013 Feb;16(2):123-8.

The Relaxant Effects of Different Methanolic Fractions of Nigella sativa on Guinea Pig Tracheal Chains.

Iranian journal of basic medical sciences

Rana Keyhanmanesh, Horeyeh Bagban, Hossein Nazemiyeh, Fariba Mirzaei Bavil, Mohammad Reza Alipour, Mehdy Ahmady

Affiliations

  1. Tuberculosis and Lung Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran.

PMID: 24298378 PMCID: PMC3843853

Abstract

OBJECTIVE(S): In regard to the high incidence of asthma and the side-effects of the drugs used, finding novel treatments for this disease is necessary. Our previous studies demonstrated the preventive effect of Nigella sativa extract on ovalbumin-induced asthma. In addition, water-soluble substances of N. sativa extract and methanol fraction of this plant were responsible for the relaxant effect of this plant on tracheal chains of guinea pigs. Therefore, for the first time, in the present study, in order to identify main constituents of the methanolic extract, the relaxant effects of five different methanolic fractions (20%, 40%, 60%, 80%, and 100%) of N. sativa on tracheal chains of guinea pigs were examined.

MATERIALS AND METHODS: The relaxant effects of four cumulative concentrations of each fraction (0.8, 1.2, 1.6, and 2.0 g%) in comparison with saline as negative control and four cumulative concentrations of theophylline (0.2, 0.4, 0.6, and 0.8 mM) were examined by their relaxant effects on precontracted tracheal chains of guinea pig by 60 mM KCl (group 1) and 10 µM methacholine (group 2).

RESULTS: In group 1, all concentrations of only theophylline showed significant relaxant effects but all concentrations of these methanolic fractions showed significant contractile effects compared with that of saline (P<0.001 to P<0.05). However, in group 2, all concentrations of theophylline and these methanolic fractions showed significant relaxant effects compared with that of saline (P<0.001 to P<0.05).

CONCLUSION: These results showed a potent relaxant effect of 20% methanolic fractions from N. sativa on tracheal chains of guinea pigs that were higher than that of theophylline at the used concentrations.

Keywords: Guinea Pig; Methanolic Fractions; Nigella sativa; Relaxant Effect; Tracheal Chain

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