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Trillmich K, Michalke W. Kinetic characterization of N-1-Naphthylphthalamic acid binding sites from maize coleoptile homogenates. Planta. 1979;145(2):119-27doi: 10.1007/BF00388707.
Trillmich, K., & Michalke, W. (1979). Kinetic characterization of N-1-Naphthylphthalamic acid binding sites from maize coleoptile homogenates. Planta, 145(2), 119-27. https://doi.org/10.1007/BF00388707
Trillmich, K, and Michalke, W. "Kinetic characterization of N-1-Naphthylphthalamic acid binding sites from maize coleoptile homogenates." Planta vol. 145,2 (1979): 119-27. doi: https://doi.org/10.1007/BF00388707
Trillmich K, Michalke W. Kinetic characterization of N-1-Naphthylphthalamic acid binding sites from maize coleoptile homogenates. Planta. 1979 Jan;145(2):119-27. doi: 10.1007/BF00388707. PMID: 24317666.
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Planta. 1979 Jan;145(2):119-27. doi: 10.1007/BF00388707.

Kinetic characterization of N-1-Naphthylphthalamic acid binding sites from maize coleoptile homogenates.

Planta

K Trillmich, W Michalke

Affiliations

  1. Institut für Biologie III der Universität Freiburg, Schänzlestraße 1, D-7800, Freiburg, Federal Republic of Germany.

PMID: 24317666 DOI: 10.1007/BF00388707

Abstract

The association of NPA with particulate material from corn coleoptile homogenates and the dissociation of the complexes formed are characterized. Using a filtration method, a single rate constant for the association at 0° C was determined as 2.7×10(-6) M(-1) min(-1), and for the dissociation of the complex a rate constant of 0.086 min(-1) was obtained with the same method. From these a dissociation constant of the complex of 3.4×10(-8) M can be calculated, which is in good agreement with the dirct determination of this constant in equilibrium measurements. This is taken as an indication for a simple reaction schema describing the reaction of NPA with its particle-bound receptor, and it can be inferred that membrane permeation does not impose a limitation on this reaction under the conditions used in this investigation. This view is strengthened by the absence of any indication for a carrier mechanism in "counterflow" experiments. A few minor deviations from quantitative predictions of the simple binding reaction schema are discussed.

References

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