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Plant Mol Biol. 1982 Dec;1(4):301-4. doi: 10.1007/BF00027561.

A simple plant polytene chromosome system, and its use for in situ hybridisation.

Plant molecular biology

D R Davies, C A Cullis

Affiliations

  1. John Innes Institute, Colney Lane, NR4 7UH, Norwich, England.

PMID: 24318036 DOI: 10.1007/BF00027561

Abstract

When the cotyledons of Pisum sativum are cultured in the presence of 2-4D, large populations of cells with polytene chromosomes are stimulated to enter prophase; the use of these chromosome for studies of in situ hybridisation with nucleic acids is described.

References

  1. Plant Physiol. 1973 Jun;51(6):1005-10 - PubMed
  2. Planta. 1976 Jan;131(3):293-8 - PubMed
  3. Exp Cell Res. 1973 Jan;76(1):175-85 - PubMed
  4. Nucleic Acids Res. 1981 Mar 25;9(6):1301-9 - PubMed

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