Planta. 1981 Oct;152(6):513-26. doi: 10.1007/BF00380822.
A light-dependent current associated with chloroplast aggregation in the alga Vaucheria sessilis.
Planta
M R Blatt, M H Weisenseel, W Haupt
Affiliations
Affiliations
- Department of Plant Biology, Carnegic Institution of Washington, 290 Panama St., 94305, Stanford, CA, USA.
PMID: 24301155
DOI: 10.1007/BF00380822
Abstract
Local irradiation of the alga Vaucheria sessilis (Vauch.) D.C. with blue light, which stimulates cortical fiber reticulation and chloroplast aggregation (M.R. Blatt and W.R. Briggs, 1980, Planta 147, 355-362), also induces an outward-directed current from the irradiated region of the cell. This current appears in conjunction with cortical fiber reticulation and precedes chloroplast aggregation. The current is not photosynthetic in origin, as indicated by experiments with 3(3,4-dichlorophenyl)-1,1-dimethyl urea and carbonyl-cyanide-m-chlorophenylhydrazone (CCCP). It shows a wavelength-dependence similar to that of chloroplast aggregation and reaches a maximum of 500 nA cm(-2) with saturating light intensities. The current is not dependent upon the presence of Na(+), K(+), or Cl(-) in a test medium containing only Na(+), K(+), Ca(2+), and Cl(-), but is inhibited, apparently nonspecifically, in the absence of external calcium. Both the light-induced current and chloroplast aggregation are stimulated by increases in the external KCl concentration and are inhibited by sub-micromolar concentrations of CCCP or by external pHs below approximately 5.5. We suggest that blue light stimulates the local extrusion of cations, possibly of protons, at the plasma membrane, an event which may act to destabilize the cortical fibers in Vaucheria, disrupt cytoplasmic streaming, and eventually lead to organelle aggregation in the light.
References
- J Membr Biol. 1974;16(2):121-44 - PubMed
- Planta. 1979 Jan;146(1):31-9 - PubMed
- Plant Physiol. 1979 Aug;64(2):165-8 - PubMed
- Planta. 1980 Jan;147(4):355-62 - PubMed
- Planta. 1980 Jan;147(4):363-75 - PubMed
- J Cell Biol. 1978 May;77(2):536-50 - PubMed
- Plant Physiol. 1979 Sep;64(3):512-8 - PubMed
- Planta. 1980 Dec;150(5):354-6 - PubMed
- J Cell Biol. 1980 Sep;86(3):885-90 - PubMed
- J Cell Biol. 1979 Dec;83(3):633-48 - PubMed
- Plant Physiol. 1978 Oct;62(4):653-61 - PubMed
- Planta. 1976 Jan;133(1):1-7 - PubMed
- Dev Biol. 1973 Dec;35(2):349-61 - PubMed
- Biochim Biophys Acta. 1977 Jan 4;464(1):179-87 - PubMed
- J Cell Biol. 1980 Aug;86(2):599-607 - PubMed
- J Cell Biol. 1979 Oct;83(1):241-8 - PubMed
- J Gen Physiol. 1974 Oct;64(4):431-42 - PubMed
- J Gen Physiol. 1968 Jul;52(1):60-87 - PubMed
- Science. 1972 May 19;176(4036):808-9 - PubMed
- Annu Rev Biochem. 1977;46:797-822 - PubMed
- J Cell Biol. 1974 Nov;63(2 Pt 1):614-28 - PubMed
- J Cell Biol. 1980 Aug;86(2):590-8 - PubMed
- Biochim Biophys Acta. 1981 Feb 12;634(2):289-308 - PubMed
- J Cell Biol. 1977 Sep;74(3):901-27 - PubMed
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