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Alam MJ, Ashraf KU. Prediction of an Epitope-based Computational Vaccine Strategy for Gaining Concurrent Immunization Against the Venom Proteins of Australian Box Jellyfish. Toxicol Int. 2013;20(3):235-53doi: 10.4103/0971-6580.121677.
Alam, M. J., & Ashraf, K. U. (2013). Prediction of an Epitope-based Computational Vaccine Strategy for Gaining Concurrent Immunization Against the Venom Proteins of Australian Box Jellyfish. Toxicology international, 20(3), 235-53. https://doi.org/10.4103/0971-6580.121677
Alam, Md Jibran, and Ashraf, Kutub Uddin Muhammad. "Prediction of an Epitope-based Computational Vaccine Strategy for Gaining Concurrent Immunization Against the Venom Proteins of Australian Box Jellyfish." Toxicology international vol. 20,3 (2013): 235-53. doi: https://doi.org/10.4103/0971-6580.121677
Alam MJ, Ashraf KU. Prediction of an Epitope-based Computational Vaccine Strategy for Gaining Concurrent Immunization Against the Venom Proteins of Australian Box Jellyfish. Toxicol Int. 2013 Sep;20(3):235-53. doi: 10.4103/0971-6580.121677. PMID: 24403734; PMCID: PMC3877492.
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Toxicol Int. 2013 Sep;20(3):235-53. doi: 10.4103/0971-6580.121677.

Prediction of an Epitope-based Computational Vaccine Strategy for Gaining Concurrent Immunization Against the Venom Proteins of Australian Box Jellyfish.

Toxicology international

Md Jibran Alam, Kutub Uddin Muhammad Ashraf

Affiliations

  1. Department of Genetic Engineering and Biotechnology, University of Chittagong, Chittagong, Bangladesh.

PMID: 24403734 PMCID: PMC3877492 DOI: 10.4103/0971-6580.121677

Abstract

BACKGROUND: Australian Box Jellyfish (C. fleckeri) has the most rapid acting venom known to in the arena of toxicological research and is capable enough of killing a person in less than 5 minutes inflicting painful, debilitating and potentially life-threatening stings in humans. It has been understood that C. fleckeri venom proteins CfTX-1, 2 and HSP70-1 contain cardiotoxic, neurotoxic and highly dermatonecrotic components that can cause itchy bumpy rash and cardiac arrest.

SUBJECTS AND METHODS: As there is no effective drug available, novel approaches regarding epitope prediction for vaccine development were performed in this study. Peptide fragments as nonamers of these antigenic venom proteins were analyzed by using computational tools that would elicit humoral and cell mediated immunity, were focused for attempting vaccine design. By ranking the peptides according to their proteasomal cleavage sites, TAP scores and IC50<250 nM, the predictions were scrutinized. Furthermore, the epitope sequences were examined by in silico docking simulation with different specific HLA receptors.

RESULTS: Interestingly, to our knowledge, this is the maiden hypothetical immunization that predicts the promiscuous epitopes with potential contributions to the tailored design of improved safe and effective vaccines against antigenic venom proteins of C. fleckeri which would be effective especially for the Australian population.

CONCLUSION: Although the computational approaches executed here are based on concrete confidence which demands more validation and in vivo experiments to validate such in silico approach.

Keywords: C. fleckeri; docking simulation; epitope prediction; vaccine design; venom proteins

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