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van der Lee J, Bald D, Kwa SL, et al. Steady-state polarized light spectroscopy of isolated Photosystem I complexes. Photosynth Res. 1993;35(3):311-21doi: 10.1007/BF00016562.
van der Lee, J., Bald, D., Kwa, S. L., van Grondelle, R., Rögner, M., & Dekker, J. P. (1993). Steady-state polarized light spectroscopy of isolated Photosystem I complexes. Photosynthesis research, 35(3), 311-21. https://doi.org/10.1007/BF00016562
van der Lee, J, et al. "Steady-state polarized light spectroscopy of isolated Photosystem I complexes." Photosynthesis research vol. 35,3 (1993): 311-21. doi: https://doi.org/10.1007/BF00016562
van der Lee J, Bald D, Kwa SL, van Grondelle R, Rögner M, Dekker JP. Steady-state polarized light spectroscopy of isolated Photosystem I complexes. Photosynth Res. 1993 Mar;35(3):311-21. doi: 10.1007/BF00016562. PMID: 24318761.
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Photosynth Res. 1993 Mar;35(3):311-21. doi: 10.1007/BF00016562.

Steady-state polarized light spectroscopy of isolated Photosystem I complexes.

Photosynthesis research

J van der Lee, D Bald, S L Kwa, R van Grondelle, M Rögner, J P Dekker

Affiliations

  1. Department of Physics and Astronomy, Vrije Universiteit, De Boelelaan 1081, 1081 HV, Amsterdam, The Netherlands.

PMID: 24318761 DOI: 10.1007/BF00016562

Abstract

Monomeric and trimeric Photosystem I core complexes from the cyanobacterium Synechocystis PCC 6803 and LHC-I containing Photosystem I (PS I-200) complexes from spinach have been characterized by steady-state, polarized light spectroscopy at 77 K. The absorption spectra of the monomeric and trimeric core complexes from Synechocystis were remarkably similar, except for the amplitude of a spectral component at long wavelength, which was about twice as large in the trimeric complexes. This spectral component did not contribute significantly to the CD-spectrum. The (77 K) steady-state emission spectra showed prominent peaks at 724 nm (for the Synechocystis core complexes) and at 735 nm (for PS I-200). A comparison of the excitation spectra of the main emission band and the absorption spectra suggested that a significant part of the excitations do not pass the red pigments before being trapped by P-700. Polarized fluorescence excitation spectra of the monomeric and trimeric core complexes revealed a remarkably high anisotropy (∼0.3) above 705 nm. This suggested one or more of the following possibilities: 1) there is one red-most pigment to which all excitations are directed, 2) there are more red-most pigments but with (almost) parallel orientations, 3) there are more red-most pigments, but they are not connected by energy transfer. The high anisotropy above 705 nm of the trimeric complexes indicated that the long-wavelength pigments on different monomers are not connected by energy transfer. In contrary to the Synechocystis core complexes, the anisotropy spectrum of the LHC I containing complexes from spinach was not constant in the region of the long-wavelength pigments, and decreased significantly below 720 nm, the wavelength where the long-wavelength pigments on the core complexes start to absorb. These results suggested that in spinach the long-wavelength pigments on core and LHC-I are connected by energy transfer and have a non-parallel average Qy(0-0) transitions.

References

  1. Biophys J. 1987 Nov;52(5):829-36 - PubMed
  2. Biochim Biophys Acta. 1992 May 20;1100(2):125-36 - PubMed
  3. Biochemistry. 1988 Aug 9;27(16):5863-9 - PubMed
  4. Plant Physiol. 1980 May;65(5):814-22 - PubMed
  5. J Biol Chem. 1990 Apr 15;265(11):6189-96 - PubMed
  6. Biophys J. 1988 May;53(5):733-45 - PubMed
  7. EMBO J. 1990 Oct;9(10):3067-75 - PubMed
  8. Biophys J. 1988 Jul;54(1):65-76 - PubMed
  9. Arch Biochem Biophys. 1974 Nov;165(1):388-97 - PubMed
  10. EMBO J. 1988 Aug;7(8):2287-93 - PubMed
  11. Biochim Biophys Acta. 1987;895(3):167-204 - PubMed
  12. FEBS Lett. 1992 Jan 13;296(1):29-32 - PubMed
  13. Biophys J. 1992 Apr;61(4):868-78 - PubMed
  14. Photosynth Res. 1992 Feb;31(2):75-87 - PubMed
  15. Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5312-6 - PubMed
  16. Biophys J. 1992 Jul;63(1):259-73 - PubMed
  17. Photosynth Res. 1991 Sep;29(3):171-5 - PubMed

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