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Klosson RJ, Krause GH. Freezing injury in cold-acclimated and unhardened spinach leaves : I. Photosynthetic reactions of thylakoids isolated from frost-damaged leaves. Planta. 1981;151(4):339-46doi: 10.1007/BF00393288.
Klosson, R. J., & Krause, G. H. (1981). Freezing injury in cold-acclimated and unhardened spinach leaves : I. Photosynthetic reactions of thylakoids isolated from frost-damaged leaves. Planta, 151(4), 339-46. https://doi.org/10.1007/BF00393288
Klosson, R J, and Krause, G H. "Freezing injury in cold-acclimated and unhardened spinach leaves : I. Photosynthetic reactions of thylakoids isolated from frost-damaged leaves." Planta vol. 151,4 (1981): 339-46. doi: https://doi.org/10.1007/BF00393288
Klosson RJ, Krause GH. Freezing injury in cold-acclimated and unhardened spinach leaves : I. Photosynthetic reactions of thylakoids isolated from frost-damaged leaves. Planta. 1981 Apr;151(4):339-46. doi: 10.1007/BF00393288. PMID: 24301976.
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Planta. 1981 Apr;151(4):339-46. doi: 10.1007/BF00393288.

Freezing injury in cold-acclimated and unhardened spinach leaves : I. Photosynthetic reactions of thylakoids isolated from frost-damaged leaves.

Planta

R J Klosson, G H Krause

Affiliations

  1. Botanisches Institut der Universität Düsseldorf, Universitätsstraße 1, D-4000, Düsseldorf 1, Germany.

PMID: 24301976 DOI: 10.1007/BF00393288

Abstract

Spinach plants (Spinacia oleracea L.) were frost-hardened by cold-acclimation to 1° C or kept in an unhardy state at 20°/14° C in phytotrons. Detached leaves were exposed to temperatures below 0°C. Rates of photosynthetic CO2 uptake by the leaves, recorded after frost treatment, served as a measure of freezing injury. Thylakoid membranes were isolated from frost-injured leaves and their photosynthetic activities tested. Ice formation occurred at about-4° to-5° C, both in unhardened and cold-acclimated leaves. After thawing, unhardened leaves appeared severely damaged when they had been exposed to-5° to-8° C. Acclimated leaves were damaged by freezing at temperatures between-10° to-14° C. The pattern of freezing damage was complex and appeared to be identical in hardened and unhardened leaves: 1. Inactivation of photosynthesis and respiration of the leaves occurred almost simultaneously. 2. When the leaves were partly damaged, the rates of photosynthetic electron transport and noncyclic photophosphorylation and the extent of light-induced H(+) uptake by the isolated thylakoids were lowered at about the same degree. The dark decay of the proton gradient was, however, not stimulated, indicating that the permeability of the membrane to-ward protons and metal cations had not increased. 3. As shown by partial reactions of the electron transport system, freezing of leaves predominantly inhibited the oxygen evolution, but photosystem II and photosystem I-dependent electron transport were also impaired. 4. Damage of the chloroplast envelope was indicated by a decline in the percentage of intact chloroplasts found in preparations from injured leaves. The results are discussed in relation to earlier studies on freezing damage of thylakoid membranes occurring in vitro.

References

  1. Biochim Biophys Acta. 1973 Apr 5;292(3):715-28 - PubMed
  2. Biochim Biophys Acta. 1975 Jul 8;396(1):72-85 - PubMed
  3. Biochim Biophys Acta. 1977 Jul 7;461(1):31-47 - PubMed
  4. Arch Biochem Biophys. 1980 Jul;202(2):546-57 - PubMed
  5. Biochim Biophys Acta. 1978 Aug 17;511(3):455-69 - PubMed
  6. Plant Physiol. 1964 Sep;39(5):712-9 - PubMed
  7. Biochim Biophys Acta. 1973 Jan 2;291(1):23-37 - PubMed
  8. Plant Physiol. 1967 Oct;42(10):1343-50 - PubMed
  9. Planta. 1977 Jan;137(3):195-201 - PubMed
  10. Eur J Biochem. 1972 Jan 31;25(1):64-70 - PubMed
  11. Biochim Biophys Acta. 1973 Apr 27;305(1):140-52 - PubMed
  12. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1095-101 - PubMed
  13. Biochim Biophys Acta. 1973 Jan 2;291(1):38-50 - PubMed
  14. J Bioenerg Biomembr. 1977 Jun;9(3):195-201 - PubMed
  15. Plant Physiol. 1978 Nov;62(5):699-705 - PubMed
  16. Biochim Biophys Acta. 1976 Jul 9;440(1):131-46 - PubMed
  17. Z Naturforsch B. 1970 Jul;25(7):718-28 - PubMed
  18. Plant Physiol. 1949 Jan;24(1):1-15 - PubMed

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