Display options
Cite
Saunders MJ, Hepler PK. Localization of membrane-associated calcium following cytokinin treatment in Funaria using chlorotetracycline. Planta. 1981;152(3):272-81doi: 10.1007/BF00385156.
Saunders, M. J., & Hepler, P. K. (1981). Localization of membrane-associated calcium following cytokinin treatment in Funaria using chlorotetracycline. Planta, 152(3), 272-81. https://doi.org/10.1007/BF00385156
Saunders, M J, and Hepler, P K. "Localization of membrane-associated calcium following cytokinin treatment in Funaria using chlorotetracycline." Planta vol. 152,3 (1981): 272-81. doi: https://doi.org/10.1007/BF00385156
Saunders MJ, Hepler PK. Localization of membrane-associated calcium following cytokinin treatment in Funaria using chlorotetracycline. Planta. 1981 Jul;152(3):272-81. doi: 10.1007/BF00385156. PMID: 24302427.
Copy Download .nbib Format: NLM
Share it on

Planta. 1981 Jul;152(3):272-81. doi: 10.1007/BF00385156.

Localization of membrane-associated calcium following cytokinin treatment in Funaria using chlorotetracycline.

Planta

M J Saunders, P K Hepler

Affiliations

  1. Botany Department, University of Massachusetts, 01003, Amherst, MA, USA.

PMID: 24302427 DOI: 10.1007/BF00385156

Abstract

We have investigated the changes in membrane-associated calcium that occur during cytokinin induced bud formation in Funaria hygrometrica Hedw. using the fluorescent Ca(2+)-chelate probe chlorotetracycline (CTC). In the target caulonema cells a localization of CTC fluorescent material becomes evident at the presumptive bud site 12 h after cytokinin treatment. By the time of the initial asymmetric division this region is four times as fluorescent as the entire caulonema cell. Bright CTC fluorescence remains localized in the dividing cells of the bud. To relate the changes in CTC fluorescence to changes in Ca(2+) as opposed to membrane-density changes we employed the general membrane marker N-phenyl-1-naphthylamine (NPN). NPN fluorescence increases only 1.5 times in the initial bud cell. We conclude that the relative amount of Ca(2+) per quantity of membrane increases in this localized area and is maintained throughout bud formation. We suggest that these increases in membrane-associated Ca(2+) indicate a localized rise in intracellular free Ca(2+) concentration brought about by cytokinin action.

References

  1. J Cell Biol. 1980 Aug;86(2):355-65 - PubMed
  2. Plant Physiol. 1973 Sep;52(3):236-9 - PubMed
  3. Int Rev Cytol. 1973;35:253-298 - PubMed
  4. Exp Cell Res. 1974 Mar 30;85(1):47-56 - PubMed
  5. J Cell Biol. 1980 Aug;86(2):490-9 - PubMed
  6. Eur J Cell Biol. 1979 Oct;20(1):51-6 - PubMed
  7. Biochem Biophys Res Commun. 1973 Oct 1;54(3):1061-6 - PubMed
  8. Nature. 1978 May 11;273(5658):149-51 - PubMed
  9. Planta. 1980 Feb;147(5):405-13 - PubMed
  10. Biochim Biophys Acta. 1973 May 25;307(3):491-512 - PubMed
  11. Int Rev Cytol. 1979;56:145-81 - PubMed
  12. Science. 1975 Dec 19;190(4220):1204-6 - PubMed
  13. Nature. 1978 Mar 16;272(5650):251-3 - PubMed
  14. Biochem Biophys Res Commun. 1971 May 7;43(3):625-30 - PubMed
  15. Plant Physiol. 1975 Apr;55(4):738-40 - PubMed
  16. Proc Natl Acad Sci U S A. 1974 May;71(5):1915-9 - PubMed
  17. Biochem Biophys Res Commun. 1973 Oct 1;54(3):1053-60 - PubMed
  18. J Cell Sci. 1975 Jul;18(2):241-56 - PubMed
  19. J Cell Biol. 1981 Mar;88(3):604-17 - PubMed
  20. Planta. 1979 Oct;146(5):615-21 - PubMed
  21. Plant Physiol. 1968 May;43(5):827-37 - PubMed

Publication Types