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Barg R, Perl M, Beckmann JS. Elevated amounts of methotrexate-binding protein, different from normal dihydrofolate reductase, in a petunia MTX(R)-cell line. Plant Mol Biol. 1987;8(1):87-94doi: 10.1007/BF00016437.
Barg, R., Perl, M., & Beckmann, J. S. (1987). Elevated amounts of methotrexate-binding protein, different from normal dihydrofolate reductase, in a petunia MTX(R)-cell line. Plant molecular biology, 8(1), 87-94. https://doi.org/10.1007/BF00016437
Barg, R, et al. "Elevated amounts of methotrexate-binding protein, different from normal dihydrofolate reductase, in a petunia MTX(R)-cell line." Plant molecular biology vol. 8,1 (1987): 87-94. doi: https://doi.org/10.1007/BF00016437
Barg R, Perl M, Beckmann JS. Elevated amounts of methotrexate-binding protein, different from normal dihydrofolate reductase, in a petunia MTX(R)-cell line. Plant Mol Biol. 1987 Jan;8(1):87-94. doi: 10.1007/BF00016437. PMID: 24302527.
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Plant Mol Biol. 1987 Jan;8(1):87-94. doi: 10.1007/BF00016437.

Elevated amounts of methotrexate-binding protein, different from normal dihydrofolate reductase, in a petunia MTX(R)-cell line.

Plant molecular biology

R Barg, M Perl, J S Beckmann

Affiliations

  1. Department of Plant Genetics and Breeding, Agricultural Research Organization, the Volcani Center, 50250, Bet Dagan, Israel.

PMID: 24302527 DOI: 10.1007/BF00016437

Abstract

A petunia cell line, 1ECB, was previously isolated by the stepwise selection procedure, for resistance to methotrexate (MTX), an antimetabolite for the enzyme dihydrofolate reductase (DHFR). Using ammonium sulfate precipitates of cell lysates of cell line 1ECB and its parental cell line (WT), it was found that the mutant has an increase of 400 fold in (3)H-MTX binding capacity and a decrease in the affinity for MTX binding, at two orders of magnitude, in comparison with the WT. In addition, the DHFR specific activity in the mutant increased only moderately (5- to 10-fold), this activity is extremely sensitive to MTX inhibition as compared to the WT. It is evident that the MTX resistance of line 1ECB results mainly from overproduction of an MTX-binding protein which differs from the WT DHFR by four biochemical criteria. This protein may serve as a trap for the excess amounts of MTX to which the cells are exposed.

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