Display options
Cite
Terrasson E, Buitink J, Righetti K, et al. An emerging picture of the seed desiccome: confirmed regulators and newcomers identified using transcriptome comparison. Front Plant Sci. 2013;4:497doi: 10.3389/fpls.2013.00497.
Terrasson, E., Buitink, J., Righetti, K., Ly Vu, B., Pelletier, S., Zinsmeister, J., Lalanne, D., & Leprince, O. (2013). An emerging picture of the seed desiccome: confirmed regulators and newcomers identified using transcriptome comparison. Frontiers in plant science, 4497. https://doi.org/10.3389/fpls.2013.00497
Terrasson, Emmanuel, et al. "An emerging picture of the seed desiccome: confirmed regulators and newcomers identified using transcriptome comparison." Frontiers in plant science vol. 4 (2013): 497. doi: https://doi.org/10.3389/fpls.2013.00497
Terrasson E, Buitink J, Righetti K, Ly Vu B, Pelletier S, Zinsmeister J, Lalanne D, Leprince O. An emerging picture of the seed desiccome: confirmed regulators and newcomers identified using transcriptome comparison. Front Plant Sci. 2013 Dec 11;4:497. doi: 10.3389/fpls.2013.00497. eCollection 2013. PMID: 24376450; PMCID: PMC3859232.
Copy Download .nbib Format: NLM
Share it on

Front Plant Sci. 2013 Dec 11;4:497. doi: 10.3389/fpls.2013.00497. eCollection 2013.

An emerging picture of the seed desiccome: confirmed regulators and newcomers identified using transcriptome comparison.

Frontiers in plant science

Emmanuel Terrasson, Julia Buitink, Karima Righetti, Benoit Ly Vu, Sandra Pelletier, Julia Zinsmeister, David Lalanne, Olivier Leprince

Affiliations

  1. Université d'Angers, UMR 1345 Institut de Recherche en Horticulture et Semences, SFR 4207 QUASAV Angers, France.
  2. Institut National de la Recherche Agronomique, UMR 1345 Institut de Recherche en Horticulture et Semences, SFR 4207 QUASAV Angers, France.
  3. Agrocampus Ouest, UMR 1345 Institut de Recherche en Horticulture et Semences, SFR 4207 QUASAV Angers, France.

PMID: 24376450 PMCID: PMC3859232 DOI: 10.3389/fpls.2013.00497

Abstract

Desiccation tolerance (DT) is the capacity to withstand total loss of cellular water. It is acquired during seed filling and lost just after germination. However, in many species, a germinated seed can regain DT under adverse conditions such as osmotic stress. The genes, proteins and metabolites that are required to establish this DT is referred to as the desiccome. It includes both a range of protective mechanisms and underlying regulatory pathways that remain poorly understood. As a first step toward the identification of the seed desiccome of Medicago truncatula, using updated microarrays we characterized the overlapping transcriptomes associated with acquisition of DT in developing seeds and the re-establishment of DT in germinated seeds using a polyethylene glycol treatment (-1.7 MPa). The resulting list contained 740 and 2829 transcripts whose levels, respectively, increased and decreased with DT. Fourty-eight transcription factors (TF) were identified including MtABI3, MtABI5 and many genes regulating flowering transition and cell identity. A promoter enrichment analysis revealed a strong over-representation of ABRE elements together with light-responsive cis-acting elements. In Mtabi5 Tnt1 insertion mutants, DT could no longer be re-established by an osmotic stress. Transcriptome analysis on Mtabi5 radicles during osmotic stress revealed that 13 and 15% of the up-regulated and down-regulated genes, respectively, are mis-regulated in the mutants and might be putative downstream targets of MtABI5 implicated in the re-establishment of DT. Likewise, transcriptome comparisons of the desiccation sensitive Mtabi3 mutants and hairy roots ectopically expressing MtABI3 revealed that 35 and 23% of the up-regulated and down-regulated genes are acting downstream of MtABI3. Our data suggest that ABI3 and ABI5 have complementary roles in DT. Whether DT evolved by co-opting existing pathways regulating flowering and cellular phase transition and cell identity is discussed.

Keywords: ABA; ABI3; ABI5; Medicago truncatula; desiccation tolerance; seed; transcription factor; transcriptome

References

  1. Philos Trans R Soc Lond B Biol Sci. 2009 Apr 27;364(1520):1059-74 - PubMed
  2. Nature. 2007 Oct 25;449(7165):1053-7 - PubMed
  3. PLoS One. 2011;6(12):e29123 - PubMed
  4. Plant J. 2006 Sep;47(5):735-50 - PubMed
  5. Curr Biol. 2011 May 10;21(9):R346-55 - PubMed
  6. Proc Natl Acad Sci U S A. 2008 Mar 18;105(11):4495-500 - PubMed
  7. Plant Physiol. 1993 Aug;102(4):1185-1191 - PubMed
  8. Plant Cell Environ. 2012 Feb;35(2):454-84 - PubMed
  9. Trends Plant Sci. 2001 Sep;6(9):431-8 - PubMed
  10. Development. 2005 Feb;132(3):603-14 - PubMed
  11. Plant J. 2001 Jun;26(6):627-35 - PubMed
  12. Cell Mol Life Sci. 2012 Oct;69(19):3175-86 - PubMed
  13. Annu Rev Plant Biol. 2010;61:651-79 - PubMed
  14. J Exp Bot. 2013 Nov;64(14):4559-73 - PubMed
  15. Plant Cell Environ. 2012 Aug;35(8):1440-55 - PubMed
  16. Plant Cell. 2003 Feb;15(2):561-76 - PubMed
  17. Plant J. 2005 Mar;41(5):697-709 - PubMed
  18. Nucleic Acids Res. 2002 Jan 1;30(1):207-10 - PubMed
  19. Plant J. 2008 Apr;54(2):205-19 - PubMed
  20. Plant J. 2007 Jul;51(1):47-59 - PubMed
  21. J Exp Bot. 2005 Aug;56(418):2119-30 - PubMed
  22. Int J Mol Sci. 2013 Mar 13;14(3):5842-78 - PubMed
  23. Plant J. 2008 Dec;56(6):891-902 - PubMed
  24. Plant Cell. 2006 Nov;18(11):2946-57 - PubMed
  25. Proc Natl Acad Sci U S A. 2012 Feb 28;109(9):3582-7 - PubMed
  26. Plant Cell. 2011 Apr;23(4):1231-48 - PubMed
  27. Physiol Plant. 2008 Oct;134(2):237-45 - PubMed
  28. J Plant Res. 2011 Jul;124(4):509-25 - PubMed
  29. J Genet Genomics. 2009 Feb;36(2):99-107 - PubMed
  30. Development. 2013 May;140(10):2118-29 - PubMed
  31. New Phytol. 2013 Jul;199(1):101-109 - PubMed
  32. Plant Cell. 2008 May;20(5):1260-77 - PubMed
  33. Plant Physiol. 2013 Jul;162(3):1706-19 - PubMed
  34. Plant Mol Biol. 2005 Sep;59(2):253-67 - PubMed
  35. Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4782-7 - PubMed
  36. Plant J. 1994 Jun;5(6):815-26 - PubMed
  37. Plant J. 2002 Nov;32(3):317-28 - PubMed
  38. Plant Mol Biol. 2006 Jan;60(1):51-68 - PubMed
  39. Plant Cell. 2004 Jul;16(7):1841-53 - PubMed
  40. Plant Physiol. 2006 Jun;141(2):446-55 - PubMed
  41. Plant Physiol. 2006 Apr;140(4):1418-36 - PubMed
  42. Plant Physiol. 2013 Aug;162(4):1768-79 - PubMed
  43. Nucleic Acids Res. 2011 Jan;39(Database issue):D1005-10 - PubMed
  44. Nature. 2005 Sep 22;437(7058):529-33 - PubMed
  45. Plant J. 2010 Mar;61(6):1041-52 - PubMed
  46. Plant Cell Rep. 2013 Jul;32(7):959-70 - PubMed
  47. New Phytol. 2011 Jul;191(1):223-233 - PubMed
  48. Plant J. 2010 Jul;63(2):212-28 - PubMed
  49. Plant Physiol. 2009 Oct;151(2):641-54 - PubMed
  50. Trends Plant Sci. 2009 Feb;14(2):110-7 - PubMed
  51. Plant Cell. 2008 Nov;20(11):3022-37 - PubMed
  52. Plant J. 2010 Mar;61(5):792-803 - PubMed
  53. Plant Cell Environ. 2007 Feb;30(2):165-75 - PubMed
  54. Plant J. 2009 Nov;60(4):614-25 - PubMed
  55. Plant Physiol. 2013 Oct;163(2):757-74 - PubMed
  56. Nucleic Acids Res. 2012 Sep 1;40(17):8240-54 - PubMed
  57. Comput Appl Biosci. 1996 Aug;12(4):357-8 - PubMed
  58. Plant Cell. 2009 Mar;21(3):736-48 - PubMed
  59. Nucleic Acids Res. 2010 Jul;38(Web Server issue):W64-70 - PubMed
  60. Naturwissenschaften. 2007 Oct;94(10):791-812 - PubMed

Publication Types