Display options
Cite
Banerjee HN, Blackshear M, Williams J, et al. C-6 Ceramide Induces p53 Dependent Apoptosis in Human Astrocytoma Grade4 (Glioblastoma Multiforme) Cells. J Cancer Sci Ther. 2012;4(2):12
Banerjee, H. N., Blackshear, M., Williams, J., Hawkins, Z., Sawyer, C., Manglik, V., & Giri, S. (2012). C-6 Ceramide Induces p53 Dependent Apoptosis in Human Astrocytoma Grade4 (Glioblastoma Multiforme) Cells. Journal of cancer science & therapy, 4(2), 12.
Banerjee, Hirendra Nath, et al. "C-6 Ceramide Induces p53 Dependent Apoptosis in Human Astrocytoma Grade4 (Glioblastoma Multiforme) Cells." Journal of cancer science & therapy vol. 4,2 (2012): 12.
Banerjee HN, Blackshear M, Williams J, Hawkins Z, Sawyer C, Manglik V, Giri S. C-6 Ceramide Induces p53 Dependent Apoptosis in Human Astrocytoma Grade4 (Glioblastoma Multiforme) Cells. J Cancer Sci Ther. 2012 Feb 01;4(2):12. PMID: 24319543; PMCID: PMC3849708.
Copy Download .nbib Format: NLM
Share it on

J Cancer Sci Ther. 2012 Feb 01;4(2):12.

C-6 Ceramide Induces p53 Dependent Apoptosis in Human Astrocytoma Grade4 (Glioblastoma Multiforme) Cells.

Journal of cancer science & therapy

Hirendra Nath Banerjee, Millon Blackshear, Jordan Williams, Zack Hawkins, Crystal Sawyer, Vinod Manglik, Shailendra Giri

Affiliations

  1. Department of Biological Sciences, Elizabethcity State University under The University of North Carolina, Elizabethcity, NC-27909.

PMID: 24319543 PMCID: PMC3849708

Abstract

Ceramide is composed of sphingosine and a fatty acid found in large concentration within the cell membrane and often acts as a signaling molecule for various functions including programmed cell death. In the present investigation, we observed that C6-ceramide induces p53-dependent apoptosis and effectively killed the Astrocytoma grade4 (Glioblastoma Multiforme) HTB12 cell lines. Ceramide-induced cell death was confirmed by Trypan blue assay which showed about 65% cells dying from ceramide treatment. Apoptosis was confirmed by Caspase3 ELISA assay and DNA fragmentation assay. The p53 induction was confirmed by immunoblot studies. Since C6 Ceramide induces apoptosis in Glioblastoma cells, it may be employed in chemotherapeutic strategy to treat this highly malignant brain cancer.

Keywords: Apoptosis; Astrocytoma; Caspase; Ceramide; ELISA; p53

References

  1. Cancer Chemother Pharmacol. 2001 May;47(5):444-50 - PubMed
  2. Brain Res. 2001 Mar 23;895(1-2):59-65 - PubMed
  3. J Biol Chem. 1997 Mar 14;272(11):6918-26 - PubMed
  4. Int J Oncol. 1999 Sep;15(3):535-40 - PubMed
  5. J Biol Chem. 2001 Aug 31;276(35):32506-14 - PubMed
  6. Melanoma Res. 1998 Jun;8(3):227-39 - PubMed
  7. Int J Oncol. 1999 Sep;15(3):541-6 - PubMed
  8. Prog Lipid Res. 2012 Jan;51(1):50-62 - PubMed
  9. J Biol Chem. 2001 Jul 6;276(27):24901-10 - PubMed
  10. FEBS Lett. 2002 Jul 3;522(1-3):161-7 - PubMed
  11. Eur J Biochem. 2003 Mar;270(5):892-901 - PubMed
  12. Biochem J. 1997 Jun 15;324 ( Pt 3):783-9 - PubMed
  13. J Pediatr Surg. 2003 Sep;38(9):1296-304 - PubMed
  14. J Clin Invest. 2011 Nov;121(11):4222-30 - PubMed
  15. Neurochem Res. 2001 Jun;26(6):667-71 - PubMed
  16. J Biol Chem. 1995 Feb 3;270(5):2047-52 - PubMed
  17. Int J Biochem Cell Biol. 2012 Apr;44(4):620-8 - PubMed
  18. Exp Cell Res. 2000 Feb 25;255(1):18-29 - PubMed
  19. J Biol Chem. 2003 Oct 3;278(40):38206-13 - PubMed
  20. Cell Death Differ. 1998 Sep;5(9):785-91 - PubMed
  21. Toxicology. 2001 Mar 7;160(1-3):97-103 - PubMed
  22. J Biol Chem. 1999 Apr 9;274(15):10654-60 - PubMed
  23. J Clin Invest. 1999 Apr;103(7):971-8 - PubMed

Publication Types

Grant support