J Diabetes Res. 2013;2013:163062. doi: 10.1155/2013/163062. Epub 2013 Nov 11.
Skeletal muscle-specific CPT1 deficiency elevates lipotoxic intermediates but preserves insulin sensitivity.
Journal of diabetes research
Wanchun Shi, Siping Hu, Wenhua Wang, Xiaohui Zhou, Wei Qiu
Affiliations
Affiliations
- Department of Endocrinology, Huzhou Central Hospital, Zhejiang 313000, China.
PMID: 24319696
PMCID: PMC3844227 DOI: 10.1155/2013/163062
Abstract
OBJECTIVE: By specific knockout of carnitine palmitoyl transferase 1b (CPT1b) in skeletal muscles, we explored the effect of CPT1b deficiency on lipids and insulin sensitivity.
METHODS: Mice with specific knockout of CPT1b in skeletal muscles (CPT1b M-/-) were used for the experiment group, with littermate C57BL/6 as controls (CPT1b). General and metabolic profiles were measured and compared between groups. mRNA expression and CPT1 activity were measured in skeletal muscle tissues and compared between groups. Mitochondrial fatty acid oxidation (FAO), triglycerides (TAGs), diglycerides (DAGs), and ceramides were examined in skeletal muscles in two groups. Phosphorylated AKT (pAkt) and glucose transporter 4 (Glut4) were determined with real-time polymerase chain reaction (RT-PCR). Insulin tolerance test, glucose tolerance test, and pyruvate oxidation were performed in both groups.
RESULTS: CPT1b M-/- model was successfully established, with impaired muscle CPT1 activity. Compared with CPT1b mice, CPT1b M-/- mice had similar food intake but lower body weight or fat mass and higher lipids but similar glucose or insulin levels. Their mitochondrial FAO of skeletal muscles was impaired. There were lipids accumulations (TAGs, DAGs, and ceramides) in skeletal muscle. However, pAkt and Glut4, insulin sensitivity, glucose tolerance, and pyruvate oxidation were preserved.
CONCLUSION: Skeletal muscle-specific CPT1 deficiency elevates lipotoxic intermediates but preserves insulin sensitivity.
References
- Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1631-40 - PubMed
- Diabetes. 2013 Feb;62(2):362-72 - PubMed
- Br J Nutr. 2013 Mar 14;109(5):810-5 - PubMed
- Biochim Biophys Acta. 2010 Jan;1801(1):1-22 - PubMed
- Alcohol Clin Exp Res. 2011 Feb;35(2):229-34 - PubMed
- Diabetes. 2013 Mar;62(3):711-20 - PubMed
- J Pharm Biomed Anal. 2012 Feb 23;60:7-13 - PubMed
- Nat Rev Endocrinol. 2011 Sep 13;8(2):92-103 - PubMed
- Diabetes. 2009 Mar;58(3):550-8 - PubMed
- J Biol Chem. 2004 Sep 24;279(39):41104-13 - PubMed
- PLoS One. 2013;8(2):e51648 - PubMed
- Physiol Genomics. 2013 May 1;45(9):367-76 - PubMed
- J Neurosci Res. 2007 Nov 1;85(14):3145-9 - PubMed
- Diabetes. 2013 Jul;62(7):2183-94 - PubMed
- J Clin Endocrinol Metab. 2013 Apr;98(4):1667-75 - PubMed
- Mol Genet Metab. 2008 Mar;93(3):314-22 - PubMed
- Diabetes. 2013 Jul;62(7):2240-8 - PubMed
- J Gastroenterol Hepatol. 2013 Aug;28(8):1403-9 - PubMed
- Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):2934-9 - PubMed
- Diabetologia. 2012 Oct;55(10):2551-2554 - PubMed
- Am J Physiol Endocrinol Metab. 2013 Jun 15;304(12):E1391-403 - PubMed
- Br J Nutr. 2013 Aug 28;110(3):447-55 - PubMed
- Endocrinol Metab Clin North Am. 2008 Sep;37(3):713-31, x - PubMed
- Circulation. 2012 Oct 2;126(14):1705-16 - PubMed
- J Clin Invest. 2005 Jul;115(7):1934-41 - PubMed
- Obesity (Silver Spring). 2011 Jan;19(1):43-8 - PubMed
- Diabete Metab. 1987 Apr;13(2):116-21 - PubMed
- Lipids Health Dis. 2012 Feb 23;11:30 - PubMed
- N Engl J Med. 2010 Mar 25;362(12):1090-101 - PubMed
- Diabetes. 2001 Jan;50(1):123-30 - PubMed
- Int J Obes (Lond). 2009 Apr;33(4):481-9 - PubMed
- Biochem Biophys Res Commun. 2013 Mar 22;432(4):553-7 - PubMed
- Obesity (Silver Spring). 2009 Jul;17(7):1396-401 - PubMed
- Rapid Commun Mass Spectrom. 2007;21(14):2361-73 - PubMed
- Diabetologia. 2013 Jul;56(7):1638-48 - PubMed
- Diabetologia. 1999 Jan;42(1):113-6 - PubMed
- Metabolism. 2011 Aug;60(8):1090-9 - PubMed
- Magn Reson Insights. 2012 Oct 15;5:29-36 - PubMed
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