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Hashemia F, Hashemian F, Bakhshaei M. The prevalence of positive fungal cultures in patients with chronic rhinosinusitis in a high altitude region of iran. Iran J Otorhinolaryngol. 2012;24(66):29-33
Hashemia, F., Hashemian, F., & Bakhshaei, M. (2012). The prevalence of positive fungal cultures in patients with chronic rhinosinusitis in a high altitude region of iran. Iranian journal of otorhinolaryngology, 24(66), 29-33.
Hashemia, Farnaz, et al. "The prevalence of positive fungal cultures in patients with chronic rhinosinusitis in a high altitude region of iran." Iranian journal of otorhinolaryngology vol. 24,66 (2012): 29-33.
Hashemia F, Hashemian F, Bakhshaei M. The prevalence of positive fungal cultures in patients with chronic rhinosinusitis in a high altitude region of iran. Iran J Otorhinolaryngol. 2012;24(66):29-33. PMID: 24303382; PMCID: PMC3846205.
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Iran J Otorhinolaryngol. 2012;24(66):29-33.

The prevalence of positive fungal cultures in patients with chronic rhinosinusitis in a high altitude region of iran.

Iranian journal of otorhinolaryngology

Farnaz Hashemia, Farshad Hashemian, Mohammadhossein Bakhshaei

Affiliations

  1. Assistant professor of Hamadan University of Medical science ,otolaryngology department.

PMID: 24303382 PMCID: PMC3846205

Abstract

INTRODUCTION: There are suspended fungal spores in the air and in the nasal mucosa of adults, especially in areas with a humid climate. Several studies have revealed the role of fungi in the pathogenesis of chronic rhinosinusitis (CRS) in recent years but it is a topic of controversy, especially in regions with low humidity. The aim of this study was to evaluate the prevalence of fungal species in intraoperative specimens from patients who underwent functional endoscopic sinus surgery (FESS) for CRS in Hamadan, a high altitude region of Iran.

MATERIALS AND METHODS: In this prospective cross-sectional study specimens were obtained from 62 patients with a diagnosis of CRS according to clinical and computed tomography criteria who underwent endoscopic sinus surgery. During the functional endoscopic sinus surgery, specimens were collected from the nose and sinuses of patients and preserved in conical centrifuge tubes containing Sputolysin and chloramphenicol. The specimens were then plated on Sabouraud dextrose agar, Mycosel agar, Niger seed agar, and Chrom Agar/Candida plates and incubated at 30°C for up to 1 month. At the end of the incubation period the samples were evaluated microscopically to detect fungi and identify their genera and species.

RESULTS: The fungal cultures were positive in 16 out of 62 patients with CRS (25.8%). In order of frequency the fungal genera and species were: Aspergillus fumigatus (9), Aspergillus niger (3), Candida albicans (2), Penicillium sp. (1) and Cladosporium sp. (1). The percentage of positive cultures collected was higher in winter but the difference was not statistically significant compared to the rest of the year.

CONCLUSION: Our data show that 25.8% of patients tested positive for the presence of fungi. The results strengthen the theory regarding the role of fungi in the pathogenesis of CRS even in areas with low humidity. Aspergillus was the most commonly isolated fungus.

Keywords: Altitude region; Chronic rhinosinusitis; Fungus

References

  1. Braz J Otorhinolaryngol. 2006 Mar-Apr;72(2):217-22 - PubMed
  2. Otolaryngol Clin North Am. 2005 Dec;38(6):1203-13 - PubMed
  3. Am J Rhinol. 2007 Sep-Oct;21(5):556-9 - PubMed
  4. Braz J Otorhinolaryngol. 2005 Nov-Dec;71(6):712-20 - PubMed
  5. Arch Environ Health. 2003 Jul;58(7):433-41 - PubMed
  6. Am J Rhinol Allergy. 2009 Nov-Dec;23(6):556-61 - PubMed
  7. Laryngoscope. 2002 Dec;112(12):2189-91 - PubMed
  8. Mayo Clin Proc. 1999 Sep;74(9):877-84 - PubMed
  9. Hum Pathol. 2004 Apr;35(4):474-81 - PubMed
  10. Otolaryngol Head Neck Surg. 2003 Sep;129(3 Suppl):S1-32 - PubMed
  11. Laryngoscope. 2003 Feb;113(2):264-9 - PubMed
  12. Auris Nasus Larynx. 2007 Jun;34(2):185-9 - PubMed

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