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Williamson FA. Concanavalin A binding sites on the plasma membrane of leek stem protoplasts. Planta. 1979;144(3):209-15doi: 10.1007/BF00388761.
Williamson, F. A. (1979). Concanavalin A binding sites on the plasma membrane of leek stem protoplasts. Planta, 144(3), 209-15. https://doi.org/10.1007/BF00388761
Williamson, F A. "Concanavalin A binding sites on the plasma membrane of leek stem protoplasts." Planta vol. 144,3 (1979): 209-15. doi: https://doi.org/10.1007/BF00388761
Williamson FA. Concanavalin A binding sites on the plasma membrane of leek stem protoplasts. Planta. 1979 Jan;144(3):209-15. doi: 10.1007/BF00388761. PMID: 24407250.
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Planta. 1979 Jan;144(3):209-15. doi: 10.1007/BF00388761.

Concanavalin A binding sites on the plasma membrane of leek stem protoplasts.

Planta

F A Williamson

Affiliations

  1. Laboratoire de Biologie Végétale, Cytologie Expérimentale, Ecole Normale Supérieure, 24 rue Lhomond, F-75231, Paris, France.

PMID: 24407250 DOI: 10.1007/BF00388761

Abstract

The binding of concanavalin A (con A) to leek (Allium porrum L.) stem protoplasts has been investigated using sequential treatment with con A and haemocyanin and using con A covalently linked to ferritin. Prefixed protoplasts were evenly labelled. Unfixed protoplasts showed a clustered distribution of label. Low temperature and lanthanum reduced the clustering. Bound con A was lost from unfixed protoplasts incubated for 5 h after treatment, but con A/haemocyanin was not bound to nascent wall materials. Prefixed protoplasts treated with wall-removing enzymes before labelling showed only a small reduction of con A binding. These results indicate that con A is bound to plasma membrane components, but that binding is reduced by competition of nascent wall precursors.

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