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Planta. 1979 Jan;144(5):467-71. doi: 10.1007/BF00380124.

Galactolipid synthesis in chromoplasts in vitro : Specificities of acyl-and galactosyltransferases.

Planta

B Liedvogel, H Kleinig

Affiliations

  1. Lehrstuhl für Zellbiologie, Institut für Biologie II der Universität, Schänzlestraße, D-7800, Freiburg/Br., Federal Republic of Germany.

PMID: 24407392 DOI: 10.1007/BF00380124

Abstract

Isolated chromoplasts from Narcissus pseudonarcissus flowers contain: a fatty acid synthesizing system; acyl-CoA synthetase (EC 6.2.1.3); glycero-phosphate acyltransferase (EC 2.3.1.15); acylglycero-phosphate acyltransferase; phosphatidate phosphatase (EC 3.1.3.4); diacylglycerol galactosyltransferase (EC 2.4.1.46); and diacylgalactosylglycerol galactosyltransferase, i.e. all enzymatic activities necessary for the synthesis of diacylgalactosylglycerol and diacylgalabiosylglycerol from acetate, HCO - (3) , sn-glycerol 3-phosphate, and UDP-D-galactose. Diacylgalactosylglycerol and diacylgalabiosylglycerol, however, are synthesized from these precursors to only a very low extent in an in vitro system. This is attributed to a specificity of diacylglycerol galactosyltransferase for highly unsaturated diacylglycerols. Specificities of acyltransferase reactions were also found.

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