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Nagaya R, Mizuno-Kamiya M, Takayama E, et al. Mechanisms of the immunosuppressive effects of mouse adipose tissue-derived mesenchymal stromal cells on mouse alloreactively stimulated spleen cells. Exp Ther Med. 2013;7(1):17-22doi: 10.3892/etm.2013.1382.
Nagaya, R., Mizuno-Kamiya, M., Takayama, E., Kawaki, H., Onoe, I., Tanabe, T., Nagahara, K., & Kondoh, N. (2014). Mechanisms of the immunosuppressive effects of mouse adipose tissue-derived mesenchymal stromal cells on mouse alloreactively stimulated spleen cells. Experimental and therapeutic medicine, 7(1), 17-22. https://doi.org/10.3892/etm.2013.1382
Nagaya, Ryo, et al. "Mechanisms of the immunosuppressive effects of mouse adipose tissue-derived mesenchymal stromal cells on mouse alloreactively stimulated spleen cells." Experimental and therapeutic medicine vol. 7,1 (2014): 17-22. doi: https://doi.org/10.3892/etm.2013.1382
Nagaya R, Mizuno-Kamiya M, Takayama E, Kawaki H, Onoe I, Tanabe T, Nagahara K, Kondoh N. Mechanisms of the immunosuppressive effects of mouse adipose tissue-derived mesenchymal stromal cells on mouse alloreactively stimulated spleen cells. Exp Ther Med. 2014 Jan;7(1):17-22. doi: 10.3892/etm.2013.1382. Epub 2013 Nov 06. PMID: 24348757; PMCID: PMC3860983.
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Exp Ther Med. 2014 Jan;7(1):17-22. doi: 10.3892/etm.2013.1382. Epub 2013 Nov 06.

Mechanisms of the immunosuppressive effects of mouse adipose tissue-derived mesenchymal stromal cells on mouse alloreactively stimulated spleen cells.

Experimental and therapeutic medicine

Ryo Nagaya, Masako Mizuno-Kamiya, Eiji Takayama, Harumi Kawaki, Ippei Onoe, Toshiichiro Tanabe, Kuniteru Nagahara, Nobuo Kondoh

Affiliations

  1. Department of Oral Implantology, Asahi University School of Dentistry, Mizuho-shi, Gifu 501-0296, Japan.
  2. Department of Oral Biochemistry, Asahi University School of Dentistry, Mizuho-shi, Gifu 501-0296, Japan.

PMID: 24348757 PMCID: PMC3860983 DOI: 10.3892/etm.2013.1382

Abstract

The mechanisms of immunomodulation by mesenchymal stromal cells remain poorly understood. In this study, the effects of mouse adipose tissue-derived mesenchymal stromal cells (ASCs) on mouse spleen cells alloreactively stimulated by anti-CD3 and anti-CD28 antibody-coated (anti-CD3/CD28) beads were observed. Production of interferon-γ by the anti-CD3/CD28 bead-stimulated spleen cells was significantly suppressed in co-culture with ASCs. However, an augmented intensity of CD69 on the stimulated spleen cells was not suppressed in the presence of ASCs. The immunosuppressive effects of ASCs were partially mediated by one or more soluble factors (26% suppression). However, the ASCs require cell-cell contact in order to maximally exert suppression (88%). The suppressive effect of ASCs mediated by direct cell contact was partially reversed following knockdown of β2 microglobulin, a component of the major histocompatibility complex (MHC) class I molecule, with siRNA. The results of the study demonstrated that ASCs have significant immune modulatory effects on alloreactively stimulated spleen cells. The effects of ASCs on spleen cells are dependent on soluble factor(s) and cell contact, which is mediated by the MHC class I complex on ASCs.

Keywords: adipose tissue-derived stromal cells; immunosuppression; interferon-γ; β2 microglobulin

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