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Diwan AD, Leong A, Appleyard R, et al. Bone morphogenetic protein-7 accelerates fracture healing in osteoporotic rats. Indian J Orthop. 2013;47(6):540-6doi: 10.4103/0019-5413.121569.
Diwan, A. D., Leong, A., Appleyard, R., Bhargav, D., Fang, Z. M., & Wei, A. (2013). Bone morphogenetic protein-7 accelerates fracture healing in osteoporotic rats. Indian journal of orthopaedics, 47(6), 540-6. https://doi.org/10.4103/0019-5413.121569
Diwan, Ashish D, et al. "Bone morphogenetic protein-7 accelerates fracture healing in osteoporotic rats." Indian journal of orthopaedics vol. 47,6 (2013): 540-6. doi: https://doi.org/10.4103/0019-5413.121569
Diwan AD, Leong A, Appleyard R, Bhargav D, Fang ZM, Wei A. Bone morphogenetic protein-7 accelerates fracture healing in osteoporotic rats. Indian J Orthop. 2013 Nov;47(6):540-6. doi: 10.4103/0019-5413.121569. PMID: 24379457; PMCID: PMC3868133.
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Indian J Orthop. 2013 Nov;47(6):540-6. doi: 10.4103/0019-5413.121569.

Bone morphogenetic protein-7 accelerates fracture healing in osteoporotic rats.

Indian journal of orthopaedics

Ashish D Diwan, Anthony Leong, Richard Appleyard, Divya Bhargav, Zhi Ming Fang, Aiqun Wei

Affiliations

  1. Orthopaedic Research Institute; Department of Orthopaedic Surgery, St. George Hospital Clinical School, University of New South Wales, Kogarah, New South Wales, Australia ; Department of Orthopaedic Surgery, St. George Hospital Clinical School, University of New South Wales, Kogarah, New South Wales, Australia.
  2. Department of Orthopaedic Surgery, St. George Hospital Clinical School, University of New South Wales, Kogarah, New South Wales, Australia.
  3. Orthopaedic Research Institute; Department of Orthopaedic Surgery, St. George Hospital Clinical School, University of New South Wales, Kogarah, New South Wales, Australia.

PMID: 24379457 PMCID: PMC3868133 DOI: 10.4103/0019-5413.121569

Abstract

BACKGROUND: Osteoporosis is characterized by low bone mass, bone fragility and increased susceptibility to fracture. Fracture healing in osteoporosis is delayed and rates of implant failure are high with few biological treatment options available. This study aimed to determine whether a single dose of bone morphogenetic protein-7 (BMP-7) in a collagen/carboxy-methyl cellulose (CMC) composite enhanced fracture healing in an osteoporotic rat model.

MATERIALS AND METHODS: An open femoral midshaft osteotomy was performed in female rats 3 months post-ovarectomy. Rats were randomized to receive either BMP-7 composite (n = 30) or composite alone (n = 30) at the fracture site during surgery. Thereafter calluses were collected on days 12, 20 and 31. Callus cross-sectional area, bone mineral density, biomechanical stiffness and maximum torque, radiographic bony union and histological callus maturity were evaluated at each time point.

RESULTS: There were statistically significant increases in bone mineral density and callus cross-section area at all time points in the BMP-7 group as compared to controls and biomechanical readings showed stronger bones at day 31 in the BMP-7 group. Histological and radiographic evaluation indicated significant acceleration of bony union in the BMP-7 group as compared to controls.

CONCLUSION: This study demonstrated that BMP-7 accelerates fracture healing in an oestrogen-deficient environment in a rat femoral fracture healing model to scientific relevance level I. The use of BMP-7 composite could offer orthopedic surgeons an advantage over oestrogen therapy, enhancing osteoporotic fracture healing with a single, locally applied dose at the time of surgery, potentially overcoming delays in healing caused by the osteoporotic state.

Keywords: Bone morphogenetic protein-7; carboxy-methyl cellulose; oestrogen; osteoporotic fracture healing; rat femur

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