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Rezaei Kanavi M, Hosseini B, Javadi F, et al. Impression cytology in eyes with clinical and confocal scan features of acanthamoeba keratitis. J Ophthalmic Vis Res. 2013;8(3):207-12
Rezaei Kanavi, M., Hosseini, B., Javadi, F., Rakhshani, N., & Javadi, M. A. (2013). Impression cytology in eyes with clinical and confocal scan features of acanthamoeba keratitis. Journal of ophthalmic & vision research, 8(3), 207-12.
Rezaei Kanavi, Mozhgan, et al. "Impression cytology in eyes with clinical and confocal scan features of acanthamoeba keratitis." Journal of ophthalmic & vision research vol. 8,3 (2013): 207-12.
Rezaei Kanavi M, Hosseini B, Javadi F, Rakhshani N, Javadi MA. Impression cytology in eyes with clinical and confocal scan features of acanthamoeba keratitis. J Ophthalmic Vis Res. 2013 Jul;8(3):207-12. PMID: 24349663; PMCID: PMC3853787.
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J Ophthalmic Vis Res. 2013 Jul;8(3):207-12.

Impression cytology in eyes with clinical and confocal scan features of acanthamoeba keratitis.

Journal of ophthalmic & vision research

Mozhgan Rezaei Kanavi, Bagher Hosseini, Fatemeh Javadi, Nasser Rakhshani, Mohammad-Ali Javadi

Affiliations

  1. Ocular Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran ; Central Eye Bank of Iran, Tehran, Iran.
  2. Central Eye Bank of Iran, Tehran, Iran.
  3. Medical Student, Shahed University of Medical Sciences, Tehran, Iran.
  4. Central Eye Bank of Iran, Tehran, Iran ; Department of Pathology, Gastrointestinal and Liver Disease Research Center, Iran University of Medical Sciences, Tehran, Iran.
  5. Central Eye Bank of Iran, Tehran, Iran ; Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

PMID: 24349663 PMCID: PMC3853787

Abstract

PURPOSE: To report impression cytology findings in specimens obtained from eyes with clinical and confocal microscopic features of Acanthamoeba keratitis (AK).

METHODS: In this interventional case series, impression cytology was obtained from corneas of patients with clinical and confocal microscopic features indicative of AK. Specimens were stained with Periodic acid-Schiff/Papanicolaou (PAS/PAP) and examined for the presence of PAS-reactive Acanthamoeba cysts and/or hyperchromatic pear-shaped trophozoites. All specimens were then decolorized and re-stained with calcofluor white (CFW) for the presence of chemofluorescent cysts.

RESULTS: Fifty-six eyes of 50 patients with mean age of 25.5±9.3 (range, 17 to 78) years were evaluated. Forty-one (82%) cases were female and 51 (91.1%) eyes had history of contact lens wear. PAS-reactive Acanthamoeba cysts and/or hyperchromatic pear-shaped trophozoites were identified in 53 eyes (94.6%), 2 of which demonstrated only trophozoite- like structures. CFW staining was able to reveal the presence of chemofluorescent cysts in all 51 specimens (91.1%) in which cysts had been demonstrated with PAS/PAP staining. Trophozoites were not detected with CFW due to background staining of the cellulose acetate strip used for impression cytology.

CONCLUSION: Corneal impression cytology, stained with PAS/PAP or with CFW, successfully detects Acanthamoeba and can be employed for early noninvasive diagnosis of AK.

Keywords: Acanthamoeba Keratitis; Confocal Scan; Impression Cytology

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