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Kim SO, Jeong HS, Jang S, et al. Spontaneous electrical activity of cultured interstitial cells of cajal from mouse urinary bladder. Korean J Physiol Pharmacol. 2013;17(6):531-6doi: 10.4196/kjpp.2013.17.6.531.
Kim, S. O., Jeong, H. S., Jang, S., Wu, M. J., Park, J. K., Jiao, H. Y., Jun, J. Y., & Park, J. S. (2013). Spontaneous electrical activity of cultured interstitial cells of cajal from mouse urinary bladder. The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology, 17(6), 531-6. https://doi.org/10.4196/kjpp.2013.17.6.531
Kim, Sun-Ouck, et al. "Spontaneous electrical activity of cultured interstitial cells of cajal from mouse urinary bladder." The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology vol. 17,6 (2013): 531-6. doi: https://doi.org/10.4196/kjpp.2013.17.6.531
Kim SO, Jeong HS, Jang S, Wu MJ, Park JK, Jiao HY, Jun JY, Park JS. Spontaneous electrical activity of cultured interstitial cells of cajal from mouse urinary bladder. Korean J Physiol Pharmacol. 2013 Dec;17(6):531-6. doi: 10.4196/kjpp.2013.17.6.531. Epub 2013 Dec 16. PMID: 24381503; PMCID: PMC3874441.
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Korean J Physiol Pharmacol. 2013 Dec;17(6):531-6. doi: 10.4196/kjpp.2013.17.6.531. Epub 2013 Dec 16.

Spontaneous electrical activity of cultured interstitial cells of cajal from mouse urinary bladder.

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology

Sun-Ouck Kim, Han-Seong Jeong, Sujeong Jang, Mei-Jin Wu, Jong Kyu Park, Han-Yi Jiao, Jae Yeoul Jun, Jong-Seong Park

Affiliations

  1. Department of Urology, Chonnam National University Medical School, Gwangju 501-757, Korea. ; Research Institute of Medical Sciences, Chonnam National University, Gwangju 501-757, Korea.
  2. Department of Physiology, Chonnam National University Medical School, Gwangju 501-757, Korea. ; Research Institute of Medical Sciences, Chonnam National University, Gwangju 501-757, Korea.
  3. Department of Physiology, Chonnam National University Medical School, Gwangju 501-757, Korea.
  4. Department of Physiology, Chonnam National University Medical School, Gwangju 501-757, Korea. ; Center for Creative Biomedical Scientists at Chonnam National Univertisity, Gwangju 501-757, Korea.
  5. Department of Neurosurgery, College of Medicine, Chosun University, Gwangju 501-759, Korea.
  6. Department of Physiology, College of Medicine, Chosun University, Gwangju 501-759, Korea.
  7. Department of Physiology, Chonnam National University Medical School, Gwangju 501-757, Korea. ; Research Institute of Medical Sciences, Chonnam National University, Gwangju 501-757, Korea. ; Center for Creative Biomedical Scientists at Chonnam National Univertisity, Gwangju 501-757, Korea.

PMID: 24381503 PMCID: PMC3874441 DOI: 10.4196/kjpp.2013.17.6.531

Abstract

Interstitial cells of Cajal (ICCs) from the urinary bladder regulate detrusor smooth muscle activities. We cultured ICCs from the urinary bladder of mice and performed patch clamp and intracellular Ca(2+) ([Ca(2+)]i) imaging to investigate whether cultured ICCs can be a valuable tool for cellular functional studies. The cultured ICCs displayed two types of spontaneous electrical activities which are similar to those recorded in intact bladder tissues. Spontaneous electrical activities of cultured ICCs were nifedipine-sensitive. Carbachol and ATP, both excitatory neurotransmitters in the urinary bladder, depolarized the membrane and increased the frequency of spike potentials. Carbachol increased [Ca(2+)]i oscillations and basal Ca(2+) levels, which were blocked by atropine. These results suggest that cultured ICCs from the urinary bladder retain rhythmic phenotypes similar to the spontaneous electrical activities recorded from the intact urinary bladder. Therefore, we suggest that cultured ICCs from the urinary bladder may be useful for cellular and molecular studies of ICCs.

Keywords: Interstitial cells of Cajal; Spontaneous electrical activities; Urinary bladder

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