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Rahmat A, Wan Ngah WZ, Gapor A, et al. Long-term tocotrienol supplementation and glutathione-dependent enzymes during hepatocarcinogenesis in the rat. Asia Pac J Clin Nutr. 1993;2(3):129-34
Rahmat, A., Wan Ngah, W. Z., Gapor, A., & Khalid, B. A. (1993). Long-term tocotrienol supplementation and glutathione-dependent enzymes during hepatocarcinogenesis in the rat. Asia Pacific journal of clinical nutrition, 2(3), 129-34.
Rahmat, A, et al. "Long-term tocotrienol supplementation and glutathione-dependent enzymes during hepatocarcinogenesis in the rat." Asia Pacific journal of clinical nutrition vol. 2,3 (1993): 129-34.
Rahmat A, Wan Ngah WZ, Gapor A, Khalid BA. Long-term tocotrienol supplementation and glutathione-dependent enzymes during hepatocarcinogenesis in the rat. Asia Pac J Clin Nutr. 1993 Sep;2(3):129-34. PMID: 24352144.
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Asia Pac J Clin Nutr. 1993 Sep;2(3):129-34.

Long-term tocotrienol supplementation and glutathione-dependent enzymes during hepatocarcinogenesis in the rat.

Asia Pacific journal of clinical nutrition

A Rahmat, W Z Wan Ngah, A Gapor, B A Khalid

Affiliations

  1. Department of Biochemistry, Faculty of Medicine, University Kebangsaan Malaysia, Kuala Lumpur, Malaysia.

PMID: 24352144

Abstract

The effects of long-term administration of tocotrienol on hepatocarcinogenesis in rats induced by diethyl nitrosamine (DEN) and 2-acetylaminofluorene (AAF) were investigated by the determination of plasma and liver gamma-glutamyl transpeptidase (GGT), cytosolic glutathione reductase (GSSG-Rx), glutathione peroxidase (GSH-Px) and glutathione S-transferase (GST). Twenty-eight male Rattus norwegicus rats (120-160g) were divided according to treatments into four groups: control group, tocotrienol - supplemented diet group (30mg/kg food), DEN/AAF-treated group and DEN/AAF treated plus tocotrienol-supplemented-diet group (30mg/kg food). The rats were sacrificed after nine months. The results obtained indicated no difference in the morphology and histology of the livers of control and tocotrienol-treated rats. Greyish-white neoplastic nodules (two per liver) were found in all the DEN/ AAF treated rats (n-10) whereas only one nodule was found in one of the carcinogen treated rats receiving tocotrienol supplementation (n-6). Histological examination showed obvious cellular damage for both the DEN/AAF-treated rats and the tocotrienol-supplemented rats but were less severe in the latter. Treatment with DEN/AAF caused increases in GGT, GSH-Px, GST and GSSG-Rx activities when compared to controls. These increases were also observed when tocotrienol was supplemented with DEN/AAF but the increases were less when compared to the rats receiving DEN/AAF only.

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