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Vagera J, Novák FJ, Vyskot B. Anther cultures of Nicotiana tabacum L. mutants. Theor Appl Genet. 1976;47(3):109-14doi: 10.1007/BF00274938.
Vagera, J., Novák, F. J., & Vyskot, B. (1976). Anther cultures of Nicotiana tabacum L. mutants. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 47(3), 109-14. https://doi.org/10.1007/BF00274938
Vagera, J, et al. "Anther cultures of Nicotiana tabacum L. mutants." TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik vol. 47,3 (1976): 109-14. doi: https://doi.org/10.1007/BF00274938
Vagera J, Novák FJ, Vyskot B. Anther cultures of Nicotiana tabacum L. mutants. Theor Appl Genet. 1976 May;47(3):109-14. doi: 10.1007/BF00274938. PMID: 24414529.
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Theor Appl Genet. 1976 May;47(3):109-14. doi: 10.1007/BF00274938.

Anther cultures of Nicotiana tabacum L. mutants.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

J Vagera, F J Novák, B Vyskot

Affiliations

  1. Institute of Experimental Botany, Czechoslovak Academy of Sciences, Experimental Station Olomouc, Praha, Czechoslovakia.

PMID: 24414529 DOI: 10.1007/BF00274938

Abstract

The theoretically expected and experimentally observed phenotypic ratios have been compared in populations of haploids derived from chlorophyll mutants of Nicotiana tabacum L. with a known genotypic constitution. The frequencies of mutant genotypes were significantly lower than the expected values, proving the existence of selection in a system of haploid embryoids developing in the anther.The anthers from M1 plants of a diploidized Nicotiana tabacum haploid cv. Samsun, treated with various concentrations of N-nitroso-N-methylurea and n-butylmethane sulphonate, were cultivated in vitro. The number of anthers which gave rise to haploids (embryogenic anthers) was stimulated by lower concentrations of both the mutagens. The stimulation at the level of M1 sporophyte is explained by internal genetic heterogeneity induced by adequate mutagen concentration. The average number of haploids per embryogenic anther decreased in all the treatments. The frequency of haploid plants of the mutant phenotypes increased with increasing mutagen concentration.

References

  1. Am J Bot. 1949 Mar;36(3):265-70 - PubMed
  2. Nature. 1965 Nov 27;208(5013):850-6 - PubMed
  3. Mutat Res. 1975 Feb;27(2):241-8 - PubMed

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